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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2004-1-21
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pubmed:abstractText |
Lipid A is the pro-inflammatory component of bacterial lipopolysaccharide, the major surface component of Gram-negative bacteria. Gram-negative bacteria alter the structure of lipid A in response to specific environmental conditions including those found upon colonization of a host. The opportunistic pathogen Pseudomonas aeruginosa synthesizes a unique hexa-acylated lipid A containing palmitate and aminoarabinose during adaptation to the cystic fibrosis airway. Different lipid A species are observed in P. aeruginosa isolated from non-cystic fibrosis associated infections. Here we report that P. aeruginosa isolates from the airway of a cystic fibrosis patient with severe pulmonary disease synthesized a novel hepta-acylated lipid A. Cystic fibrosis-specific P. aeruginosa lipid A modifications result in resistance to host antimicrobial peptides and increased recognition by human Toll-like receptor 4 (TLR4). Using P. aeruginosa lipid A with different levels of acylation, we identified a 222 amino acid region in the extracellular portion of human TLR4 that is required for the differential recognition of cystic fibrosis-specific lipid A. P. aeruginosa adaptation to the human airway may, therefore, play a fundamental role in the progressive lung damage associated with cystic fibrosis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0968-0519
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
395-400
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pubmed:dateRevised |
2011-5-5
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pubmed:meshHeading |
pubmed-meshheading:14733728-Amino Acid Sequence,
pubmed-meshheading:14733728-Cell Line,
pubmed-meshheading:14733728-Cystic Fibrosis,
pubmed-meshheading:14733728-Genetic Variation,
pubmed-meshheading:14733728-Humans,
pubmed-meshheading:14733728-Kidney,
pubmed-meshheading:14733728-Lipid A,
pubmed-meshheading:14733728-Lipopolysaccharides,
pubmed-meshheading:14733728-Mass Spectrometry,
pubmed-meshheading:14733728-Membrane Glycoproteins,
pubmed-meshheading:14733728-Molecular Weight,
pubmed-meshheading:14733728-Pseudomonas aeruginosa,
pubmed-meshheading:14733728-Receptors, Cell Surface,
pubmed-meshheading:14733728-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:14733728-Toll-Like Receptor 4,
pubmed-meshheading:14733728-Toll-Like Receptors,
pubmed-meshheading:14733728-Transfection
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pubmed:year |
2003
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pubmed:articleTitle |
Pseudomonas aeruginosa lipid A diversity and its recognition by Toll-like receptor 4.
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pubmed:affiliation |
Department of Medicine, HSB K-155, Box 357710, University of Washington, Seattle, WA 98195, USA. rkernst@u.washington.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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