Linked Life Data

14729267

Source:http://linkedlifedata.com/resource/pubmed/id/14729267

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-1-19
pubmed:databankReference
pubmed:abstractText
Protein synthesis elongation factor 2 (eEF2) catalyzes the translocation of the peptidyl-tRNA from the A site to the P site of the ribosome. Most organisms encode a single EF2 protein and its activity is regulated by phosphorylation. We have identified a family of genes in Tetrahymena thermophila that encode proteins homologous to eEF2, yet are expressed only during sexual reproduction. These genes have been designated EFR for Elongation Factor 2 Related. EFR transcripts were not detected in vegetative cell cultures but rapidly increased about 6 h after the start of conjugation (mating). For comparison, we cloned, sequenced and analyzed the expression of the standard eEF2 gene from T. thermophila. Unlike EFR, transcripts from eEF2 were detected in vegetative cells but were present at lower concentrations during conjugation. Despite the high sequence identity between EFR and eEF2 from other organisms (about 42% at the amino acid level), key regulatory sequences that are involved in the regulation of eEF2 are altered in EFR. The sequence and expression data suggest that EFR is an eEF2 variant involved in a major translation regulatory mechanism that occurs during the formation of the macronuclear genome in conjugating cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
326
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
97-105
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14729267-Amino Acid Sequence, pubmed-meshheading:14729267-Animals, pubmed-meshheading:14729267-Cloning, Molecular, pubmed-meshheading:14729267-DNA, Protozoan, pubmed-meshheading:14729267-Gene Expression Regulation, Developmental, pubmed-meshheading:14729267-Molecular Sequence Data, pubmed-meshheading:14729267-Peptide Elongation Factor 2, pubmed-meshheading:14729267-Peptide Elongation Factors, pubmed-meshheading:14729267-Protozoan Proteins, pubmed-meshheading:14729267-RNA, Protozoan, pubmed-meshheading:14729267-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:14729267-Sequence Alignment, pubmed-meshheading:14729267-Sequence Analysis, DNA, pubmed-meshheading:14729267-Sequence Homology, Amino Acid, pubmed-meshheading:14729267-Tetrahymena thermophila, pubmed-meshheading:14729267-Transcription, Genetic
pubmed:year
2004
pubmed:articleTitle
Identification of a developmentally regulated translation elongation factor 2 in Tetrahymena thermophila.
pubmed:affiliation
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't