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rdf:type |
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lifeskim:mentions |
umls-concept:C0003593,
umls-concept:C0007634,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0033684,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0087063,
umls-concept:C0332298,
umls-concept:C0444930,
umls-concept:C2239176
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pubmed:issue |
2-3
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pubmed:dateCreated |
2004-1-19
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pubmed:abstractText |
Bile salts (BS) inhibit the secretion of apolipoprotein B (apoB) and triacylglycerol (TG) in primary rat, mouse and human hepatocytes and in mice in vivo. We investigated whether lipidation of apoB into a lipoprotein particle is required for this inhibitory action of BS. The sodium/taurocholate co-transporting polypeptide (Ntcp) was co-expressed in McArdle-RH7777 (McA-RH7777) cells stably expressing the full-length human apoB100 (h-apoB100, secreted as TG-rich lipoprotein particles) or carboxyl-truncated human apoB18 (h-apoB18, secreted in lipid-free form). The doubly transfected cell lines (h-apoB/r-Ntcp) effectively accumulated taurocholic acid (TC). TC incubation decreased the secretion of endogenous rat apoB100 (-50%) and h-apoB18 (-35%), but did not affect secretion of rat apoA-I. Pulse-chase experiments (35S-methionine) indicated that the impaired secretion of radiolabeled h-apoB18 and h-apoB100 was associated with accelerated intracellular degradation. The calpain protease inhibitor N-acetyl-leucyl-leucyl-norleucinal (ALLN) partially inhibited intracellular apoB degradation but did not affect the amount of either h-apoB18 or h-apoB100 secreted into the medium, indicating that inhibition of apoB secretion by TC is not due to calpain-dependent proteasomal degradation. We conclude that TC does not inhibit apoB secretion by interference with its lipidation, but rather involves a mechanism dependent on the N-terminal end of apoB.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein A-I,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoprotein B-100,
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins B,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Leupeptins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, VLDL,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Anion Transporters...,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Taurocholic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Triglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/acetylleucyl-leucyl-norleucinal,
http://linkedlifedata.com/resource/pubmed/chemical/sodium-bile acid cotransporter,
http://linkedlifedata.com/resource/pubmed/chemical/very low density lipoprotein...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
1635
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-103
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14729072-Animals,
pubmed-meshheading:14729072-Apolipoprotein A-I,
pubmed-meshheading:14729072-Apolipoprotein B-100,
pubmed-meshheading:14729072-Apolipoproteins B,
pubmed-meshheading:14729072-Carrier Proteins,
pubmed-meshheading:14729072-Cell Line, Tumor,
pubmed-meshheading:14729072-Cysteine Proteinase Inhibitors,
pubmed-meshheading:14729072-Hepatocytes,
pubmed-meshheading:14729072-Leupeptins,
pubmed-meshheading:14729072-Lipoproteins, VLDL,
pubmed-meshheading:14729072-Liver Neoplasms, Experimental,
pubmed-meshheading:14729072-Membrane Transport Proteins,
pubmed-meshheading:14729072-Organic Anion Transporters, Sodium-Dependent,
pubmed-meshheading:14729072-Rats,
pubmed-meshheading:14729072-Symporters,
pubmed-meshheading:14729072-Taurocholic Acid,
pubmed-meshheading:14729072-Time Factors,
pubmed-meshheading:14729072-Transfection,
pubmed-meshheading:14729072-Triglycerides
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pubmed:year |
2003
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pubmed:articleTitle |
Inhibition of apolipoprotein B secretion by taurocholate is controlled by the N-terminal end of the protein in rat hepatoma McArdle-RH7777 cells.
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pubmed:affiliation |
Department of Pediatrics, Groningen University Institute for Drug Exploration, Pediatric Gastroenterology, Academic Hospital, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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