Linked Life Data

14727177

Source:http://linkedlifedata.com/resource/pubmed/id/14727177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-3-1
pubmed:abstractText
Mechanisms for the removal of glutamate are vital for maintaining normal function of the retina. Five excitatory amino acid transporters have been characterized to date from neuronal tissue, all of which are expressed within the retina except excitatory amino acid transporter 4 (EAAT4). In this study we examined the expression and localization of the glutamate transporter EAAT4 in the rat retina using RT-PCR and immunocytochemistry. RT-PCR using rat EAAT4 specific primers revealed a prominent 296-bp product in the retina, cortex and cerebellum. The identity of the EAAT4 fragment was confirmed by DNA sequencing. We examined the tissue expression levels of EAAT4 in cortex, retina and cerebellum using real-time PCR. The highest expression level was found in the cerebellum. Expression in the cortex was approximately 3.1% that of the cerebellum and the retina was found to have approximately 0.8% the total cerebellar EAAT4 content. In order to examine the specific cell types within the retina that express EAAT4, we performed immunocytochemistry using a rat EAAT4 specific antiserum. Cellular processes within the nerve fibre layer of the retina were intensely labelled for EAAT4. Double labelling EAAT4 with glial fibrillary acidic protein (GFAP) revealed extensive colocalization indicating that EAAT4 is localized within astrocytes within the retina. Double labelling of EAAT4 and the glutamate transporter EAAT1 (GLAST) revealed extensive colocalization suggesting that astrocytes in the retina express at least two types of glutamate transporters. These results suggest that astrocytes within the retina are well placed to provide mechanisms for glutamate removal as well as controlling cellular excitability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
315
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
305-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14727177-Amino Acid Transport System X-AG, pubmed-meshheading:14727177-Animals, pubmed-meshheading:14727177-Astrocytes, pubmed-meshheading:14727177-Excitatory Amino Acid Transporter 1, pubmed-meshheading:14727177-Excitatory Amino Acid Transporter 4, pubmed-meshheading:14727177-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:14727177-Gene Expression, pubmed-meshheading:14727177-Glial Fibrillary Acidic Protein, pubmed-meshheading:14727177-Glutamate Plasma Membrane Transport Proteins, pubmed-meshheading:14727177-Microscopy, Confocal, pubmed-meshheading:14727177-RNA, Messenger, pubmed-meshheading:14727177-Rats, pubmed-meshheading:14727177-Retina, pubmed-meshheading:14727177-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:14727177-Sequence Analysis, DNA, pubmed-meshheading:14727177-Symporters
pubmed:year
2004
pubmed:articleTitle
Localization and expression of the glutamate transporter, excitatory amino acid transporter 4, within astrocytes of the rat retina.
pubmed:affiliation
Department of Anatomy and Cell Biology, The University of Melbourne, Grattan St., 3010 Parkville, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't