Linked Life Data

1472711

Source:http://linkedlifedata.com/resource/pubmed/id/1472711

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-1-29
pubmed:databankReference
pubmed:abstractText
To clone and sequence the cDNA of the growth hormone of European sea bass (Dicentrarchus labrax L.) (sbGH), total pituitary RNA was reverse transcribed and amplified using polymerase chain reaction (PCR). Degenerate oligonucleotides, designed by comparing available GH cDNA sequences from related teleost species, were used as primers to amplify the 5' end and the core region of sbGH cDNA, while the 3' end was amplified according to the Rapid Amplification of cDNA ends (RACE) method. SbGH cDNA contains an open reading frame encoding a preprotein of 204 amino acids. The deduced amino acid sequence shows a putative signal peptide of 17 amino acids, suggesting that the mature hormone consists of 187 amino acids. Sequence comparison indicates a high degree of conservation of GH cDNAs within the Percoidei infraorder. Our procedure based on degenerate oligonucleotides and PCR provides a straightforward approach to clone GH cDNAs from other related bony fishes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1042-5179
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:geneSymbol
sbGH
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
185-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Cloning and sequencing of European sea bass (Dicentrarchus labrax L.) growth hormone cDNA using polymerase chain reaction and degenerate oligonucleotides.
pubmed:affiliation
Department of Biology, University of Padova, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't