|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2004-5-28
|
|
pubmed:abstractText |
To obtain a novel matrix metalloproteinase (MMP) inhibitor produced by bacteria, we have focused on the chelating activity of siderophores. Several siderophore-producing bacteria were isolated from soil using chrome azurol S agar plates and then the effect of siderophores on MMP-2 activity was assayed by gelatin zymography. The results showed that partially purified siderophores from ten isolated strains inhibited MMP-2 activity. Among these strains, two were non-fluorescent and eight were fluorescent Pseudomonas species. From these eight strains, pyoverdine-type siderophores were detected. The Zn(2+)-chelating activity of these siderophores correlated with the inhibition of MMP-2 activity. Therefore, it is considered that siderophores such as pyoverdines inhibit MMP-2 activity by chelating Zn(2+) on the active site of MMP-2.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0175-7598
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
64
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
840-7
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:14727091-Binding Sites,
pubmed-meshheading:14727091-Chromatography, Liquid,
pubmed-meshheading:14727091-Hydroxybenzoic Acids,
pubmed-meshheading:14727091-Matrix Metalloproteinase 2,
pubmed-meshheading:14727091-Oligopeptides,
pubmed-meshheading:14727091-Pseudomonas,
pubmed-meshheading:14727091-Siderophores,
pubmed-meshheading:14727091-Soil Microbiology,
pubmed-meshheading:14727091-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:14727091-Zinc
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Inhibition of matrix metalloproteinase-2 activity by siderophores of Pseudomonas species.
|
|
pubmed:affiliation |
Institute of Applied Biochemistry, University of Tsukuba, 305-8572 Tsukuba, Ibaraki, Japan.
|
|
pubmed:publicationType |
Journal Article
|
