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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0017428,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0086222,
umls-concept:C0086860,
umls-concept:C0185117,
umls-concept:C0205054,
umls-concept:C0678594,
umls-concept:C0936012,
umls-concept:C1705733,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
2004-1-16
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pubmed:abstractText |
The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcription factor. The hepatic expression of SHARP-2 mRNA was investigated under various conditions. The level was decreased in the regenerating rat liver and malignant hepatoma cells. In contrast, the expression of SHARP-2 mRNA was induced in rat livers by feeding a high-carbohydrate diet. To analyze the molecular mechanism involved in the regulation of the rat SHARP-2 gene expression, the gene was cloned. It was approximately 6-kb in length and consists of five exons and four introns. To investigate the transcriptional regulatory region of this gene, SHARP-2/firefly luciferase reporter plasmids were transfected into hepatoma cells. A functional analysis of 5(')-deletion constructs revealed that two E box sequences between -160 and -144 are mainly responsible for promoter activity. Although upstream stimulatory factors (USFs) bound to the element in vitro, USF2 failed to stimulate promoter activity from the element using the co-transfection experiment. Therefore, other E box-binding transcription factors differing from USF proteins or USF-associated proteins are necessary for transcriptional stimulation of the rat SHARP-2 gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix...,
http://linkedlifedata.com/resource/pubmed/chemical/Bhlhb2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Upstream Stimulatory Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Usf1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Usf2 protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0003-9861
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
422
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
81-90
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:14725860-Animals,
pubmed-meshheading:14725860-Base Sequence,
pubmed-meshheading:14725860-Basic Helix-Loop-Helix Transcription Factors,
pubmed-meshheading:14725860-Cell Line, Tumor,
pubmed-meshheading:14725860-DNA-Binding Proteins,
pubmed-meshheading:14725860-Gene Components,
pubmed-meshheading:14725860-Gene Deletion,
pubmed-meshheading:14725860-Gene Expression Regulation,
pubmed-meshheading:14725860-Genes,
pubmed-meshheading:14725860-Hepatocytes,
pubmed-meshheading:14725860-Homeodomain Proteins,
pubmed-meshheading:14725860-Liver,
pubmed-meshheading:14725860-Luciferases,
pubmed-meshheading:14725860-Male,
pubmed-meshheading:14725860-Molecular Sequence Data,
pubmed-meshheading:14725860-Oligonucleotides,
pubmed-meshheading:14725860-Promoter Regions, Genetic,
pubmed-meshheading:14725860-RNA, Messenger,
pubmed-meshheading:14725860-Rats,
pubmed-meshheading:14725860-Rats, Sprague-Dawley,
pubmed-meshheading:14725860-Transcription, Genetic,
pubmed-meshheading:14725860-Transcription Factors,
pubmed-meshheading:14725860-Upstream Stimulatory Factors
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pubmed:year |
2004
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pubmed:articleTitle |
The rat enhancer of split- and hairy-related protein-2 gene: hepatic expression, genomic structure, and promoter analysis.
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pubmed:affiliation |
Department of Pediatrics, Fukui Medical University, 910-1193 Fukui, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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