|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2004-1-28
|
|
pubmed:abstractText |
Dendritic cells (DCs) ingest antigens in peripheral tissues and migrate to lymph nodes where they present MHC class II-bound antigen to CD4(+) T cells. We used two-photon microscopy to image the single-cell dynamics of interactions between DCs and T cells within intact lymph nodes in the absence of relevant antigen. DCs were fluorescently labeled in vivo by cutaneous injection of alum adjuvant including carboxyfluorescein diacetate succinimidyl ester (CFSE). CFSE-positive DCs (CD11c(+), CD11b(+), and low-to-intermediate CD8(+)) were observed in draining lymph nodes 24-72 h later. Labeled DCs meandered slowly (2-3 microm x min(-1)) in the T cell zone near B cell follicles but vigorously extended long agile dendrites. Encounters between T cells and DCs arose as T cells moved autonomously along random paths. Moreover, T cells did not accumulate around DCs, and their relative velocities approaching and departing DCs were equivalent, implying that T cells are not attracted toward DCs by chemotactic gradients but rather encounter them by chance. T cell/DC contacts occurred primarily on dendrites at arm's length from the DC soma and typically lasted approximately 3 min, enabling an individual DC to interact with up to 5000 T cells per hour. We conclude that dynamic DC gesticulation and random T cell motility together enhance the stochastic scanning of the T cell repertoire, thereby enabling rapid initiation of the immune response.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-11018170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-11175800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-11342632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-11561183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12016203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12052961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12176895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12193689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12397360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12415310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12496473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12601158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12730692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12871638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12888794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12900266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-12953093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-2115981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-2118680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-2212694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-4573839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-6475812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-8176234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14722354-9653094
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
27
|
|
pubmed:volume |
101
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
998-1003
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
T cell repertoire scanning is promoted by dynamic dendritic cell behavior and random T cell motility in the lymph node.
|
|
pubmed:affiliation |
Department of Physiology and Biophysics , University of California, Irvine, CA 92697, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
