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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
2004-1-14
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pubmed:abstractText |
The stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to 45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture plastic and collagen I; and 59% +/- 7% on Matrigel. Lipid volume surpassed 4.5 x 10(3) microm3 cell(-1) for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident for Matrigel and fibronectin, and cell spreading was most pronounced for fibronectin with a projected area exceeding 3 x 10(3) microm2 cell(-1). These results are relevant to the design of an adipose implant, providing insight into its feasibility and scaffold composition.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0141-5492
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1967-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14719807-Adipocytes,
pubmed-meshheading:14719807-Adult,
pubmed-meshheading:14719807-Breast,
pubmed-meshheading:14719807-Cell Culture Techniques,
pubmed-meshheading:14719807-Cell Differentiation,
pubmed-meshheading:14719807-Cell Division,
pubmed-meshheading:14719807-Cells, Cultured,
pubmed-meshheading:14719807-Collagen,
pubmed-meshheading:14719807-Collagen Type I,
pubmed-meshheading:14719807-Drug Combinations,
pubmed-meshheading:14719807-Endothelium, Vascular,
pubmed-meshheading:14719807-Extracellular Matrix,
pubmed-meshheading:14719807-Feasibility Studies,
pubmed-meshheading:14719807-Female,
pubmed-meshheading:14719807-Fibronectins,
pubmed-meshheading:14719807-Humans,
pubmed-meshheading:14719807-Laminin,
pubmed-meshheading:14719807-Lipids,
pubmed-meshheading:14719807-Middle Aged,
pubmed-meshheading:14719807-Proteoglycans,
pubmed-meshheading:14719807-Stromal Cells,
pubmed-meshheading:14719807-Tissue Engineering
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pubmed:year |
2003
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pubmed:articleTitle |
Extracellular matrix substrata alter adipocyte yield and lipogenesis in primary cultures of stromal-vascular cells from human adipose.
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pubmed:affiliation |
Department of Chemical Engineering, Tulane University, Boggs Center, Room 300, New Orleans, LA 70118, USA. koc@tulane.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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