Source:http://linkedlifedata.com/resource/pubmed/id/14715641
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
2004-4-20
|
| pubmed:abstractText |
The Stro-1 antigen potentially defines a mesenchymal stem cell (MSC) progenitor subset. We here report on the role of human ex vivo-expanded selected Stro-1(+) or Stro-1(-) MSC subsets on the engraftment of human CD34(+) cord blood cells in the nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mouse model. The data show that cotransplantation of expanded Stro-1(-) cells with CD34(+) cells resulted in a significant increase of human CD45, CD34, CD19, and CD11b cells detected in blood or in bone marrow (BM) and spleen as compared with the infusion of CD34(+) cells alone. Infusion into mice of expanded Stro-1(+) and Stro-1(-) cells (without CD34(+) cells) showed that the numbers of Stro-1(+)-derived (as assessed by DNA analysis of human beta-globin with quantitative polymerase chain reaction [PCR]) were higher than Stro-1(-)-derived cells in spleen, muscles, BM, and kidneys, while more Stro-1(-)-derived than Stro-1(+)-derived cells were found in lungs. The transduction of expanded Stro-1(+) cells with an enhanced green fluorescent protein (eGFP) gene did not modify their cytokine release and their homing in NOD/SCID mouse tissues. The difference between the hematopoietic support and the homing capabilities of expanded Stro-1(+) and Stro-1(-) cells may be of importance for clinical therapeutic applications: Stro-1(+) cells may rather be used for gene delivery in tissues while Stro-1(-) cells may rather be used to support hematopoietic engraftment.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-4971
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| pubmed:author |
pubmed-author:AigueperseJocelyneJ,
pubmed-author:BensidhoumMoradM,
pubmed-author:BerthoJean MarcJM,
pubmed-author:BouchetSandrineS,
pubmed-author:ChapelAlainA,
pubmed-author:CharbordPierreP,
pubmed-author:DemarquayChristelleC,
pubmed-author:FouillardLoicL,
pubmed-author:FrancoisSabineS,
pubmed-author:GorinNorbert ClaudeNC,
pubmed-author:GourmelonPatrickP,
pubmed-author:LopezManuelM,
pubmed-author:MazurierChristelleC,
pubmed-author:ThierryDominiqueD
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
103
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3313-9
|
| pubmed:dateRevised |
2007-4-14
|
| pubmed:meshHeading |
pubmed-meshheading:14715641-Animals,
pubmed-meshheading:14715641-Bone Marrow Cells,
pubmed-meshheading:14715641-Cell Movement,
pubmed-meshheading:14715641-Cells, Cultured,
pubmed-meshheading:14715641-Cord Blood Stem Cell Transplantation,
pubmed-meshheading:14715641-Graft Survival,
pubmed-meshheading:14715641-Hematopoiesis,
pubmed-meshheading:14715641-Humans,
pubmed-meshheading:14715641-Mesenchymal Stem Cell Transplantation,
pubmed-meshheading:14715641-Mesenchymal Stem Cells,
pubmed-meshheading:14715641-Mice,
pubmed-meshheading:14715641-Mice, SCID,
pubmed-meshheading:14715641-Models, Animal,
pubmed-meshheading:14715641-Nuclear Proteins,
pubmed-meshheading:14715641-Organ Specificity,
pubmed-meshheading:14715641-Spleen,
pubmed-meshheading:14715641-Transplantation, Heterologous
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment.
|
| pubmed:affiliation |
Laboratoire de Thérapie Cellulaire et de Radioprotection Accidentelle, Faculté de Médecine Saint Antoine et IRSN, EA 1638 et Inserm U76, Paris, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|