Linked Life Data

14715535

Source:http://linkedlifedata.com/resource/pubmed/id/14715535

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-3-5
pubmed:abstractText
Mutations in human cardiac calsequestrin (CASQ2), a high-capacity calcium-binding protein located in the sarcoplasmic reticulum (SR), have recently been linked to effort-induced ventricular arrhythmia and sudden death (catecholaminergic polymorphic ventricular tachycardia). However, the precise mechanisms through which these mutations affect SR function and lead to arrhythmia are presently unknown. In this study, we explored the effect of adenoviral-directed expression of a canine CASQ2 protein carrying the catecholaminergic polymorphic ventricular tachycardia-linked mutation D307H (CASQ2(D307H)) on Ca2+ signaling in adult rat myocytes. Total CASQ2 protein levels were consistently elevated approximately 4-fold in cells infected with adenoviruses expressing either wild-type CASQ2 (CASQ2(WT)) or CASQ2(D307H). Expression of CASQ2(D307H) reduced the Ca2+ storing capacity of the SR. In addition, the amplitude, duration, and rise time of macroscopic I(Ca)-induced Ca2+ transients and of spontaneous Ca2+ sparks were reduced significantly in myocytes expressing CASQ2(D307H). Myocytes expressing CASQ2(D307H) also displayed drastic disturbances of rhythmic oscillations in [Ca2+]i and membrane potential, with signs of delayed afterdepolarizations when undergoing periodic pacing and exposed to isoproterenol. Importantly, normal rhythmic activity was restored by loading the SR with the low-affinity Ca2+ buffer, citrate. Our data suggest that the arrhythmogenic CASQ2(D307H) mutation impairs SR Ca2+ storing and release functions and destabilizes the Ca2+-induced Ca2+ release mechanism by reducing the effective Ca2+ buffering inside the SR and/or by altering the responsiveness of the Ca2+ release channel complex to luminal Ca2+. These results establish at the cellular level the pathological link between CASQ2 mutations and the predisposition to adrenergically mediated arrhythmias observed in patients carrying CASQ2 defects.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1524-4571
pubmed:author
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
471-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:14715535-Adenoviridae, pubmed-meshheading:14715535-Amino Acid Substitution, pubmed-meshheading:14715535-Animals, pubmed-meshheading:14715535-Calcium Signaling, pubmed-meshheading:14715535-Calsequestrin, pubmed-meshheading:14715535-Death, Sudden, Cardiac, pubmed-meshheading:14715535-Dogs, pubmed-meshheading:14715535-Genetic Vectors, pubmed-meshheading:14715535-Humans, pubmed-meshheading:14715535-Ion Channel Gating, pubmed-meshheading:14715535-Macromolecular Substances, pubmed-meshheading:14715535-Male, pubmed-meshheading:14715535-Mutation, Missense, pubmed-meshheading:14715535-Myocytes, Cardiac, pubmed-meshheading:14715535-Point Mutation, pubmed-meshheading:14715535-Rats, pubmed-meshheading:14715535-Rats, Sprague-Dawley, pubmed-meshheading:14715535-Recombinant Fusion Proteins, pubmed-meshheading:14715535-Ryanodine Receptor Calcium Release Channel, pubmed-meshheading:14715535-Sarcoplasmic Reticulum, pubmed-meshheading:14715535-Tachycardia, Ventricular
pubmed:year
2004
pubmed:articleTitle
Abnormal calcium signaling and sudden cardiac death associated with mutation of calsequestrin.
pubmed:affiliation
Department of Physiology, Texas Tech University Health Sciences Center, Lubbock, Tex 79430-6551, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't