Linked Life Data

14710595

Source:http://linkedlifedata.com/resource/pubmed/id/14710595

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2004-1-8
pubmed:abstractText
The trichothecene mycotoxins, Shiga toxins (STs), and ricin are potent translational inhibitors that exert diverse mechanisms of action but all have the capacity to induce death by apoptosis. Germinal centers containing actively dividing B cells are particularly sensitive to protein synthesis inhibition, and, of these, the immature B cell is reportedly most susceptible to apoptosis. The objective of this study was to test the hypothesis that immature and mature B-cell lineages were differentially susceptible to apoptosis and cytotoxicity induction by representative trichothecene mycotoxins, ST-1, and ricin, as well as cycloheximide (CHX), a prototypical protein synthesis inhibitor commonly used to study cell signal transduction. WEHI-231 and CH31 cells were used as representatives of phenotypically immature B cells, whereas CH12.LX cells were used to model mature B cells. Resultant data suggest that Type D and Type A trichothecenes, ricin, and ST-1 were more potent inducers of apoptosis than CHX, whereas Type B and Type A trichothecene metabolites were less. CHX and the trichothecenes affected immature and mature B cells equally, thus suggesting that toxicity due to these natural toxins was lineage independent. In contrast, mature B cells were more sensitive to ricin- and ST-1-induced cytotoxicity and apoptosis than immature B cells. Taken together, these results suggest that B cells are targets of a diverse array of naturally occurring translational inhibitors. Upregulation of apoptosis in B lymphocytes may contribute to the impairment of the immune response and other symptoms described following exposure to these toxins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1528-7394
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2105-18
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Comparative susceptibility of B cells with different lineages to cytotoxicity and apoptosis induction by translational inhibitors.
pubmed:affiliation
Department of Food Science and Human Nutrition, Institute for Environmental Toxicology, Michigan State University, East Lansing, MI 48824-1224, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't