Source:http://linkedlifedata.com/resource/pubmed/id/14709353
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2004-1-7
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| pubmed:abstractText |
Atherosclerosis has a close relationship to inflammation, particularly T helper type 1 lymphocyte (Th1) response. Interleukin-10 (IL-10), is thought to suppress Th1 response. To target therapeutic strategy for atherosclerosis, we tested whether IL-10 gene transfer suppresses atherosclerosis in apolipoprotein E-knockout (apoE-KO) mice. Four-week-old apoE-KO mice were divided into two groups and either murine IL-10 cDNA plasmid or empty control vector was transferred to the femoral muscle with the use of Hemagglutinating virus of Japan (HVJ)-liposome. At 1 week after transfection, high cholesterol diet was started and continued for 8 weeks. After euthanasia, histological studies of atherosclerotic lesions and quantitative RT-PCR for Th1 cytokines (IL-12 and IFN-gamma) in spleens were performed. IL-10 cDNA gene transfer to the muscle increased plasma IL-10 levels and depressed expression of Th1 cytokines without changing plasma cholesterol levels. IL-10 gene transfer significantly reduced the atherosclerotic plaque area and the macrophage infiltrated area. IL-12 and IFN-gamma mRNA expressions in spleens and plasma IFN-gamma levels were decreased by IL-10 gene transfer. Therefore, IL-10 gene transfer changed the Th1 response and suppressed atherosclerotic lesion formation in apoE-KO mice. IL-10 could be a new target as a therapeutic tool for the treatment of atherosclerosis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apolipoproteins E,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
|
| pubmed:issn |
0021-9150
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| pubmed:author |
pubmed-author:HirataKen-IchiK,
pubmed-author:InoueNobutakaN,
pubmed-author:KanedaYasufumiY,
pubmed-author:KawashimaSeinosukeS,
pubmed-author:MorishitaRyuichiR,
pubmed-author:NamikiMasayukiM,
pubmed-author:OzakiMasanoriM,
pubmed-author:SakodaTsuyoshiT,
pubmed-author:YamashitaTomoyaT,
pubmed-author:YokoyamaMitsuhiroM
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| pubmed:issnType |
Print
|
| pubmed:volume |
172
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
21-9
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:14709353-Animals,
pubmed-meshheading:14709353-Apolipoproteins E,
pubmed-meshheading:14709353-Arteriosclerosis,
pubmed-meshheading:14709353-DNA, Complementary,
pubmed-meshheading:14709353-Gene Therapy,
pubmed-meshheading:14709353-Gene Transfer Techniques,
pubmed-meshheading:14709353-Genetic Vectors,
pubmed-meshheading:14709353-Interferon-gamma,
pubmed-meshheading:14709353-Interleukin-10,
pubmed-meshheading:14709353-Interleukin-12,
pubmed-meshheading:14709353-Liposomes,
pubmed-meshheading:14709353-Male,
pubmed-meshheading:14709353-Mice,
pubmed-meshheading:14709353-Mice, Knockout,
pubmed-meshheading:14709353-Plasmids,
pubmed-meshheading:14709353-RNA, Messenger,
pubmed-meshheading:14709353-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14709353-Sendai virus
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| pubmed:year |
2004
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| pubmed:articleTitle |
Intramuscular gene transfer of interleukin-10 cDNA reduces atherosclerosis in apolipoprotein E-knockout mice.
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| pubmed:affiliation |
Department of Internal Medicine, Division of Cardiovascular and Respiratory Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, 650-0017, Kobe, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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