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rdf:type |
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lifeskim:mentions |
umls-concept:C0003241,
umls-concept:C0005955,
umls-concept:C0043240,
umls-concept:C0151763,
umls-concept:C0205314,
umls-concept:C0302189,
umls-concept:C0333668,
umls-concept:C0374711,
umls-concept:C0679622,
umls-concept:C1257890,
umls-concept:C1705181
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pubmed:issue |
4
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pubmed:dateCreated |
2004-1-6
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pubmed:abstractText |
We previously reported a new in vivo model named as "GFP/CCl(4) model" for monitoring the transdifferentiation of green fluorescent protein (GFP) positive bone marrow cell (BMC) into albumin-positive hepatocyte under the specific "niche" made by CCl(4) induced persistent liver damage, but the subpopulation which BMCs transdifferentiate into hepatocytes remains unknown. Here we developed a new monoclonal antibody, anti-Liv8, using mouse E 11.5 fetal liver as an antigen. Anti-Liv8 recognized both hematopoietic progenitor cells in fetal liver at E 11.5 and CD45-positive hematopoietic cells in adult bone marrow. We separated Liv8-positive and Liv8-negative cells and then transplanted these cells into a continuous liver damaged model. At 4 weeks after BMC transplantation, more efficient repopulation and transdifferentiation of BMC into hepatocytes were seen with Liv8-negative cells. These findings suggest that the subpopulation of Liv8-negative cells includes useful cells to perform cell therapy on repair damaged liver.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:KatadaToshiakiT,
pubmed-author:MiyamotoKojiK,
pubmed-author:NishinaHiroshiH,
pubmed-author:OhataShinyaS,
pubmed-author:OkitaKiwamuK,
pubmed-author:OmoriKaoruK,
pubmed-author:SakaidaIsaoI,
pubmed-author:ShinodaKohK,
pubmed-author:TeraiShujiS,
pubmed-author:WatanabeTomomiT,
pubmed-author:YamamotoNaokiN
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
313
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1110-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:14706657-Albumins,
pubmed-meshheading:14706657-Animals,
pubmed-meshheading:14706657-Antibodies, Monoclonal,
pubmed-meshheading:14706657-Antigens, CD45,
pubmed-meshheading:14706657-Bone Marrow Cells,
pubmed-meshheading:14706657-Bone Marrow Transplantation,
pubmed-meshheading:14706657-Carbon Tetrachloride,
pubmed-meshheading:14706657-Cell Differentiation,
pubmed-meshheading:14706657-Cell Separation,
pubmed-meshheading:14706657-Female,
pubmed-meshheading:14706657-Fetus,
pubmed-meshheading:14706657-Green Fluorescent Proteins,
pubmed-meshheading:14706657-Hematopoietic Stem Cells,
pubmed-meshheading:14706657-Hepatocytes,
pubmed-meshheading:14706657-Liver,
pubmed-meshheading:14706657-Luminescent Proteins,
pubmed-meshheading:14706657-Mice,
pubmed-meshheading:14706657-Mice, Inbred C57BL,
pubmed-meshheading:14706657-Mice, Knockout,
pubmed-meshheading:14706657-Mice, Transgenic,
pubmed-meshheading:14706657-Phenotype,
pubmed-meshheading:14706657-Recombinant Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
A subpopulation of bone marrow cells depleted by a novel antibody, anti-Liv8, is useful for cell therapy to repair damaged liver.
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pubmed:affiliation |
Department of Molecular Science and Applied Medicine (Gastroenterology and Hepatology), Yamaguchi University School of Medicine, Minami Kogushi 1-1-1, Ube, Yamaguchi 755-8505, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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