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rdf:type |
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lifeskim:mentions |
umls-concept:C0001128,
umls-concept:C0001675,
umls-concept:C0006104,
umls-concept:C0008466,
umls-concept:C0011602,
umls-concept:C0013935,
umls-concept:C0020205,
umls-concept:C0039005,
umls-concept:C1149299,
umls-concept:C1524075,
umls-concept:C1709707,
umls-concept:C2266866,
umls-concept:C2700400
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pubmed:issue |
11
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pubmed:dateCreated |
2004-3-8
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pubmed:abstractText |
We have shown that over-sulfated chondroitin sulfate/dermatan sulfate (CS/DS) chains from various marine organisms exhibit growth factor binding activities and neurite outgrowth-promoting activities in embryonic mouse hippocampal neurons in vitro. In this study we demonstrated that CS/DS hybrid chains purified from embryonic pig brain displayed marked neuritogenic activity and growth factor binding activities toward fibroblast growth factor 2 (FGF2), FGF10, FGF18, pleiotrophin, and midkine, all of which exhibit neuroregulatory activities in the brain. In contrast, the CS/DS preparation from adult pig brain showed considerably less activity to bind these growth factors and no neuritogenic activity. Structural analysis indicated that the average size of the CS/DS chains was similar (40 kDa) between these two preparations, but the disaccharide compositions differed considerably, with a significant proportion of l-iduronic acid (IdoUA)-containing disaccharides (8 approximately 9%) in the CS/DS chains from embryos but not in those from adults (<1%). Interestingly, both neurite outgrowth-promoting activity and growth factor binding activities of the CS/DS chains from embryos were abolished by digestion not only with chondroitinase ABC but also with chondroitinase B, suggesting that the IdoUA-containing motifs are essential for these activities. These findings imply that the temporal expression of CS/DS hybrid structures containing both GlcUA and IdoUA and binding activities toward various growth factors play important roles in neurogenesis in the early stages of the development of the brain.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chondroitin Sulfates,
http://linkedlifedata.com/resource/pubmed/chemical/Dermatan Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Disaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Fgf10 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 10,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronates,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Hyaluronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Iduronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/fibroblast growth factor 18
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9765-76
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14699094-Amino Acid Motifs,
pubmed-meshheading:14699094-Animals,
pubmed-meshheading:14699094-Binding Sites,
pubmed-meshheading:14699094-Biosensing Techniques,
pubmed-meshheading:14699094-Brain,
pubmed-meshheading:14699094-Cells, Cultured,
pubmed-meshheading:14699094-Chondroitin Sulfates,
pubmed-meshheading:14699094-Chromatography, High Pressure Liquid,
pubmed-meshheading:14699094-Dermatan Sulfate,
pubmed-meshheading:14699094-Disaccharides,
pubmed-meshheading:14699094-Dose-Response Relationship, Drug,
pubmed-meshheading:14699094-Fibroblast Growth Factor 10,
pubmed-meshheading:14699094-Fibroblast Growth Factor 2,
pubmed-meshheading:14699094-Fibroblast Growth Factors,
pubmed-meshheading:14699094-Glucuronates,
pubmed-meshheading:14699094-Growth Substances,
pubmed-meshheading:14699094-Heparin,
pubmed-meshheading:14699094-Hippocampus,
pubmed-meshheading:14699094-Hyaluronic Acid,
pubmed-meshheading:14699094-Iduronic Acid,
pubmed-meshheading:14699094-Kinetics,
pubmed-meshheading:14699094-Mice,
pubmed-meshheading:14699094-Neurons,
pubmed-meshheading:14699094-Protein Binding,
pubmed-meshheading:14699094-Recombinant Fusion Proteins,
pubmed-meshheading:14699094-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:14699094-Swine,
pubmed-meshheading:14699094-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Chondroitin sulfate/dermatan sulfate hybrid chains from embryonic pig brain, which contain a higher proportion of L-iduronic acid than those from adult pig brain, exhibit neuritogenic and growth factor binding activities.
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pubmed:affiliation |
Department of Biochemistry, Kobe Pharmaceutical University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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