Linked Life Data

14698632

Source:http://linkedlifedata.com/resource/pubmed/id/14698632

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-12-30
pubmed:abstractText
In vitro transcription/translation systems are becoming more useful to produce proteins and determine the protein function. Expression of proteins in vitro provides a rapid and controllable system to express proteins. The ability to control the system by addition of other compounds has allowed for the production of proteins that had been difficult to produce by conventional expression systems. More recently, the development of continuous in vitro systems has allowed the production of larger quantities of proteins for downstream purification techniques. Also, the use of different cell lysates allows for specific post-translational modifications of the synthesized proteins. The ability to label the translated proteins can aid structural analysis and allow tracking of specific proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1046-2023
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
191-8
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Serpin production using rapid in vitro transcription/translation systems.
pubmed:affiliation
Division of Newborn Medicine, Children's Hospital Boston, Harvard Medical School, 300 Longwood Ave, Boston, MA 02115, USA. clifford.luke@tch.harvard.edu
pubmed:publicationType
Journal Article