Linked Life Data

14698104

Source:http://linkedlifedata.com/resource/pubmed/id/14698104

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-12-30
pubmed:abstractText
Penicillium nalgiovense is a filamentous fungus that is acquiring increasing biotechnological importance in the food industry due to its widespread use as starter culture for cured and fermented meat products. Strains of P. nalgiovense can be improved by genetic modification to remove the production of penicillin and other potentially hazardous secondary metabolites, to improve its capacity to control the growth of undesirable fungi and bacteria on the meat product, and other factors that contribute to the ripening of the product in order to get safer and better quality foods. Genetic manipulation of P. nalgiovense has been limited by the lack of molecular genetics tools that were available for this fungus, particularly for "self-cloning" avoiding the use of exogenous DNAs. In this article we describe a series of vectors, selectable markers and transformation methods that can be used for efficient transformation of P. nalgiovense, gene cloning and expression. A uridine auxotrophic P. nalgiovense mutant with an inactive pyrG gene has been isolated. The P. nalgiovense wild-type pyrG gene was cloned and sequenced, and vectors carrying the gene were shown to complement the pyrG mutant. Autonomously replicating plasmids carrying the AMA1 region from Aspergillus nidulans transformed P. nalgiovense very efficiently; these plasmids were shown to be maintained as stable extrachromosomal elements in P. nalgiovense and could be rescued in Escherichia coli. The mitotic stability of self-replicative AMA1 plasmids in P. nalgiovense was higher than that reported for Penicillium chrysogenum.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0168-1605
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-48
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
High efficiency transformation of Penicillium nalgiovense with integrative and autonomously replicating plasmids.
pubmed:affiliation
Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, Avda. del Real, no. 1, 24006, León, Spain,
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't