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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1993-1-25
|
| pubmed:abstractText |
High yields of toxic shock syndrome toxin-1, from Staphylococcus aureus, have been purified (> 99%) using a novel, simple, two-step procedure involving dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the orthorhombic space group C222(1), with unit cell dimensions a = 108.6 A, b = 177.6 A and c = 97.5 A, with three molecules per asymmetric unit. The crystals diffract to at least 2.5 A resolution and are suitable for three-dimensional X-ray structural analysis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0022-2836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
228
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
983-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1469731-Bacterial Toxins,
pubmed-meshheading:1469731-Chromatography, Affinity,
pubmed-meshheading:1469731-Crystallization,
pubmed-meshheading:1469731-Enterotoxins,
pubmed-meshheading:1469731-Staphylococcus aureus,
pubmed-meshheading:1469731-Superantigens,
pubmed-meshheading:1469731-X-Ray Diffraction
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Purification, crystallization and preliminary X-ray analysis of toxic shock syndrome toxin-1 from Staphylococcus aureus.
|
| pubmed:affiliation |
Department of Biochemistry, University of Bath, Avon, U.K.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|