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rdf:type |
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lifeskim:mentions |
umls-concept:C0001272,
umls-concept:C0030685,
umls-concept:C0205100,
umls-concept:C0205171,
umls-concept:C0205276,
umls-concept:C0391871,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C1283071,
umls-concept:C1521990,
umls-concept:C1963578,
umls-concept:C2327527
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pubmed:issue |
1 Pt 1
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pubmed:dateCreated |
2003-12-25
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pubmed:abstractText |
Ca2+ release from the endoplasmic reticulum (ER) contributes to Ca2+ transients in frog sympathetic ganglion neurons. Here we use video-rate confocal fluo-4 fluorescence imaging to show that single action potentials reproducibly trigger rapidly rising Ca2+ transients at 1-3 local hot spots within the peripheral ER-rich layer in intact neurons in fresh ganglia and in the majority (74%) of cultured neurons. Hot spots were located near the nucleus or the axon hillock region. Other regions exhibited either slower and smaller signals or no response. Ca2+ signals spread into the cell at constant velocity across the ER in nonnuclear regions, indicating active propagation, but spread with a (time)1/2 dependence within the nucleus, consistent with diffusion. 26% of cultured cells exhibited uniform Ca2+ signals around the periphery, but hot spots were produced by loading the cytosol with EGTA or by bathing such cells in low-Ca2+ Ringer's solution. Peripheral hot spots for Ca2+ release within the perinuclear and axon hillock regions provide a mechanism for preferential initiation of nuclear and axonal Ca2+ signals by single action potentials in sympathetic ganglion neurons.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-10051512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-10091003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-10230787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-10811724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-10851183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-10858665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11007880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11032777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11055997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11055998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11124983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11162855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11423394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-11429446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-13943314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-1648178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-1648939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-7612818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-7716515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-7738847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-7816933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-7834745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-8229800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-8235594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-8772440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-8865063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9000075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9041445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9080362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9292729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9295386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9384593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9808459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14695260-9925818
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14695260-Action Potentials,
pubmed-meshheading:14695260-Animals,
pubmed-meshheading:14695260-Calcium,
pubmed-meshheading:14695260-Calcium Signaling,
pubmed-meshheading:14695260-Cells, Cultured,
pubmed-meshheading:14695260-Ganglia, Sympathetic,
pubmed-meshheading:14695260-Neurons,
pubmed-meshheading:14695260-Rana pipiens,
pubmed-meshheading:14695260-Tissue Distribution
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pubmed:year |
2004
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pubmed:articleTitle |
Peripheral hot spots for local Ca2+ release after single action potentials in sympathetic ganglion neurons.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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