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rdf:type |
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lifeskim:mentions |
umls-concept:C0011900,
umls-concept:C0023827,
umls-concept:C0030415,
umls-concept:C0032520,
umls-concept:C0035647,
umls-concept:C0220825,
umls-concept:C0336791,
umls-concept:C0520510,
umls-concept:C0694873,
umls-concept:C0812222,
umls-concept:C1266129,
umls-concept:C1521871
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pubmed:issue |
1
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pubmed:dateCreated |
2003-12-24
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pubmed:abstractText |
Atypical lipomatous tumours/well-differentiated liposarcomas and dedifferentiated liposarcomas are characterized by 12q13-15 region amplification. In contrast, this molecular event has not been reported in benign lipomas. Within the 12q13-15 chromosomal region, the MDM2, SAS, HMGA2, and CDK4 genes are the most frequent targets of amplification. A series of lipomas (36 cases) and liposarcomas (48 cases) was analysed for MDM2 and CDK4 gene amplification by real-time PCR. MDM2 and CDK4 gene amplification was detected in 2.8% and 5.6% of lipomas and 98.2% and 82.4% of liposarcomas, respectively. Moreover, co-amplification of the two genes as well as a higher-level amplification was observed more frequently in dedifferentiated liposarcomas than in atypical lipomatous tumours/well-differentiated liposarcomas. Real-time PCR proved to be a fast and reliable method to characterize lipomas and liposarcomas by quantification of MDM2 and CDK4 gene amplification. It is applicable to paraffin wax-embedded tissues and could be useful when histological diagnosis is difficult.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDK4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/MDM2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-mdm2
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-3417
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2004 John Wiley & Sons, Ltd.
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pubmed:issnType |
Print
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pubmed:volume |
202
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
95-102
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14694526-Abdominal Neoplasms,
pubmed-meshheading:14694526-Adult,
pubmed-meshheading:14694526-Aged,
pubmed-meshheading:14694526-Cell Line, Tumor,
pubmed-meshheading:14694526-Cyclin-Dependent Kinase 4,
pubmed-meshheading:14694526-Cyclin-Dependent Kinases,
pubmed-meshheading:14694526-DNA, Neoplasm,
pubmed-meshheading:14694526-Extremities,
pubmed-meshheading:14694526-Female,
pubmed-meshheading:14694526-Gene Amplification,
pubmed-meshheading:14694526-Head and Neck Neoplasms,
pubmed-meshheading:14694526-Humans,
pubmed-meshheading:14694526-Lipoma,
pubmed-meshheading:14694526-Liposarcoma,
pubmed-meshheading:14694526-Male,
pubmed-meshheading:14694526-Middle Aged,
pubmed-meshheading:14694526-Nuclear Proteins,
pubmed-meshheading:14694526-Paraffin Embedding,
pubmed-meshheading:14694526-Polymerase Chain Reaction,
pubmed-meshheading:14694526-Proto-Oncogene Proteins,
pubmed-meshheading:14694526-Proto-Oncogene Proteins c-mdm2,
pubmed-meshheading:14694526-Retroperitoneal Neoplasms
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pubmed:year |
2004
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pubmed:articleTitle |
Evaluation of MDM2 and CDK4 amplification by real-time PCR on paraffin wax-embedded material: a potential tool for the diagnosis of atypical lipomatous tumours/well-differentiated liposarcomas.
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pubmed:affiliation |
Department of Pathology, Institut Bergonié, Bordeaux, France. hostein@bergonie.org
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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