Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1993-1-27
pubmed:abstractText
Transport systems involved in uptake and biliary secretion of bile salts have been extensively studied in rat liver; however, little is known about these systems in the human liver. In this study, we investigated taurocholate (TC) transport in canalicular and basolateral plasma membrane vesicles isolated from 15 human livers (donor age 6-64 yr). ATP stimulated the uptake of TC into both canalicular and basolateral human liver plasma membrane vesicles (cLPM and blLPM, respectively). Considerable interindividual variations in the transport velocity were observed in the different membrane preparations used: 9.0 +/- 1.3 (mean +/- SEM, n = 17; range 1.6-18.0) and 9.3 +/- 2.0 (range 1.1-29.8) pmol TC.mg protein-1.min-1 at 1.0 microM TC for cLPM and blLPM, respectively. TC transport was temperature sensitive and showed saturation kinetics with a high affinity for TC (Km 4.2 +/- 0.7 microM and 3.7 +/- 0.5 microM for cLPM and blLPM, respectively). Transport was dependent on the ATP concentration and saturable (Km 0.25 +/- 0.03 mM, n = 3). Neither nitrate, which reduces membrane potential, nor the protonophore FCCP strongly inhibited ATP-dependent TC transport, indicating that membrane potential and proton gradient are not involved in this process. TC transport was significantly inhibited by the classical anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonate (250 microM) and the glutathione conjugate S-(2,4-dinitrophenyl)glutathione (100 microM). In conclusion, high affinity ATP-dependent TC transport is present in human liver at both the canalicular and the basolateral sides of the hepatocyte.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-13193360, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-13511347, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-14317604, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-1531002, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-1862084, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-1916666, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-1918007, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-1932051, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2025276, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2071597, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2172249, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2185668, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-219124, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2322307, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2333302, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2333997, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2335503, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2440891, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2463674, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2568355, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2760217, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2777205, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-2894550, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-3191999, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-3345203, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-3366909, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-3413083, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-3593261, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-3654750, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-4018730, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-6469975, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-6630516, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-6707198, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-7118069, http://linkedlifedata.com/resource/pubmed/commentcorrection/1469089-7176836
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2321-6
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Adenosine triphosphate-dependent taurocholate transport in human liver plasma membranes.
pubmed:affiliation
Department of Pediatrics, University of Groningen, The Netherlands.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't