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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-12-22
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pubmed:abstractText |
Escherichia coli Rsd protein was previously identified on the basis of its binding to the RNA polymerase sigma(70) subunit. The Rsd-sigma(70) complex has been studied using different methods. Our data show that Rsd associates with sigma(70) to form a complex with a stoichiometry of 1:1. Alanine scanning and deletion mutagenesis were used to locate regions of sigma(70) that are required for the formation of the Rsd-sigma(70) complex.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-2836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
335
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
685-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14687566-Binding Sites,
pubmed-meshheading:14687566-DNA-Directed RNA Polymerases,
pubmed-meshheading:14687566-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:14687566-Escherichia coli Proteins,
pubmed-meshheading:14687566-Molecular Weight,
pubmed-meshheading:14687566-Mutagenesis, Site-Directed,
pubmed-meshheading:14687566-Protein Binding,
pubmed-meshheading:14687566-Repressor Proteins,
pubmed-meshheading:14687566-Sigma Factor,
pubmed-meshheading:14687566-Spectrometry, Mass, Electrospray Ionization
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pubmed:year |
2004
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pubmed:articleTitle |
Studies of the Escherichia coli Rsd-sigma70 complex.
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pubmed:affiliation |
School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. westbll@rockefeller.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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