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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0030705,
umls-concept:C0040028,
umls-concept:C0301625,
umls-concept:C0522310,
umls-concept:C0547047,
umls-concept:C0694891,
umls-concept:C0870509,
umls-concept:C1510411,
umls-concept:C1524003
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pubmed:issue |
2
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pubmed:dateCreated |
2003-12-22
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pubmed:abstractText |
Essential thrombocythaemia (ET) is characterized by the abnormal and sustained proliferation of megakaryocytes. The mechanism for this lineage-specific expansion in ET, remains unclear. We have previously reported that transforming growth factor-beta1 (TGF-beta1) is involved in negative feedback regulation of megakaryopoiesis in both healthy volunteers (HV) and patients with idiopathic thrombocytopenic purpura (ITP). The present study found that megakaryocyte colony-forming units (CFU-MK) of ET patients were less sensitive to TGF-beta1 than those of HV. The expression of Smad4 (Sma- and Mad-related protein-4) in CFU-MK of ET patients was reduced in comparison with that of HV. Finally, to confirm that the impaired TGF-beta1 sensitivity was caused by reduced expression of Smad4, we examined Smad4-transfected CFU-MK from ET patients in the presence of TGF-beta1, and verified that the transfectants were indeed as susceptible as CFU-MK from HV to TGF-beta1. Thus it was surmised that one of the mechanisms for impaired sensitivity of CFU-MK to TGF-beta1 is the reduced expression of Smad4.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SMAD2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SMAD3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SMAD4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Smad2 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Smad3 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Smad4 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0007-1048
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pubmed:author |
pubmed-author:FujikawaKoshiK,
pubmed-author:HirayamaYasuoY,
pubmed-author:KatoJunjiJ,
pubmed-author:KohdaKyuheiK,
pubmed-author:KurodaHiroyukiH,
pubmed-author:MatsunagaTakuyaT,
pubmed-author:NiitsuYoshiroY,
pubmed-author:SakamakiSumioS,
pubmed-author:TakayamaTetsujiT,
pubmed-author:TakimotoRishuR,
pubmed-author:TanakaIkutaI,
pubmed-author:TeruiTakeshiT
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pubmed:issnType |
Print
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pubmed:volume |
124
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
211-20
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:14687032-Adult,
pubmed-meshheading:14687032-Aged,
pubmed-meshheading:14687032-Aged, 80 and over,
pubmed-meshheading:14687032-DNA-Binding Proteins,
pubmed-meshheading:14687032-Dose-Response Relationship, Drug,
pubmed-meshheading:14687032-Female,
pubmed-meshheading:14687032-Gene Expression,
pubmed-meshheading:14687032-Genetic Vectors,
pubmed-meshheading:14687032-Humans,
pubmed-meshheading:14687032-Male,
pubmed-meshheading:14687032-Megakaryocytes,
pubmed-meshheading:14687032-Middle Aged,
pubmed-meshheading:14687032-RNA, Messenger,
pubmed-meshheading:14687032-Retroviridae,
pubmed-meshheading:14687032-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14687032-Signal Transduction,
pubmed-meshheading:14687032-Smad2 Protein,
pubmed-meshheading:14687032-Smad3 Protein,
pubmed-meshheading:14687032-Smad4 Protein,
pubmed-meshheading:14687032-Thrombocythemia, Essential,
pubmed-meshheading:14687032-Trans-Activators,
pubmed-meshheading:14687032-Transfection,
pubmed-meshheading:14687032-Transforming Growth Factor beta,
pubmed-meshheading:14687032-Transforming Growth Factor beta1
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pubmed:year |
2004
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pubmed:articleTitle |
Decrease of Smad4 gene expression in patients with essential thrombocythaemia may cause an escape from suppression of megakaryopoiesis by transforming growth factor-beta1.
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pubmed:affiliation |
Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan.
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pubmed:publicationType |
Journal Article
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