Linked Life Data

14678516

Source:http://linkedlifedata.com/resource/pubmed/id/14678516

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2003-12-17
pubmed:abstractText
Rapid freezing of cell monolayers at ambient pressure is limited regarding the thickness of ice crystal damage-free freezing. The specific freezing conditions of the cells under investigation are decisive for the success of such methods. Improved reproducibility of results could be expected by cryoimmobilization at high pressure because this achieves a greater thickness of adequate freezing. In a novel approach, we tested the suitability of sapphire discs as cell substrata for high-pressure freezing. Frozen samples on sapphire were subjected to freeze-substitution while in the same flat sample holders as used for high-pressure freezing. We obtained cells that displayed an excellent preservation of fine structure. Because sapphire is a tissue culture substratum suitable for light microscopy, its use in combination with high-pressure freezing could become a powerful tool in correlative studies of cell dynamics at light and electron microscopic levels.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-2720
pubmed:author
pubmed:issnType
Print
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
High-pressure freezing of epithelial cells on sapphire coverslips.
pubmed:affiliation
Institute of Biochemistry and Molecular Cell Biology, University of Vienna, Vienna Biocenter, 1030 Vienna, Austria. ulstr@abc.univie.ac.at
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't