Linked Life Data

14676358

Source:http://linkedlifedata.com/resource/pubmed/id/14676358

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-12-16
pubmed:abstractText
The A6 epithelial cell line, derived from the kidney of Xenopus laevis, spontaneously form domes after it has reached confluence. In a previous study, we demonstrated that formation of domes is strongly inhibited in the cells cultured using a three-dimensional clinostat. In this study, we performed staining of filamentous actin and examination using electron microscopy to investigate morphological changes of A6 cells exposured to clinorotation for 10 days. Micrographs show that A6 cells in clinorotation lose cortical actin that is characteristic of epithelial cells. Therefore, we search for genes differentially expressed in A6 cells cultured in clinorotation and identified Xenopus laevis N-myc downstream-regulated gene-1 (xNDRG1) as a clinorotation respondent gene in A6 cells. In northern blots analysis, xNDRG1 mRNA significantly increased only in A6 cells cultured in clinorotation for 10 days, and maintained at a similar level in the cells cultured for 15 days. Centrifugations of A6 cells have no effect on expression of xNDRG1. We also aimed to characterize xNDRG1 during Xenopus laevis development by examining the temporal and spatial expression patterns of xNDRG1 transcripts in embryos. Our results suggest that xNDRG1 is necessary for pronephros development in Xenopus laevis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
S
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0914-9201
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NASA
pubmed:authorsComplete
Y
pubmed:pagination
171-2
pubmed:dateRevised
2009-6-4
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Characterization of a gene respondent to clinorotation in Xenopus A6 cells.
pubmed:affiliation
Department of Life Sciences (Biology), Graduate School of Arts and Sciences, University of Tokyo.
pubmed:publicationType
Journal Article