Linked Life Data

14675444

Source:http://linkedlifedata.com/resource/pubmed/id/14675444

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-12-16
pubmed:abstractText
The molecular mechanisms of plant responses to iron (Fe) deficiency remain largely unknown. To identify the cis-acting elements responsible for Fe-deficiency-inducible expression in higher plants, the barley IDS2 (iron deficiency specific clone no. 2) gene promoter was analyzed using a transgenic tobacco system. Deletion analysis revealed that the sequence between -272 and -91 from the translational start site (-272/-91) was both sufficient and necessary for specific expression in tobacco roots. Further deletion and linker-scanning analysis of this region clearly identified two cis-acting elements: iron-deficiency-responsive element 1 (IDE1) at -153/-136 (ATCAAGCATGCTTCTTGC) and IDE2 at -262/-236 (TTGAACGGCAAGTTTCACGCTGTCACT). The co-existence of IDE1 and IDE2 was essential for specific expression when the -46/+8 region (relative to the transcriptional start site) of the CaMV 35S promoter was used as a minimal promoter. Expression occurred mainly in the root pericycle, endodermis, and cortex. When the -90/+8 region of the CaMV 35S promoter was fused, the -272/-227 region, which consists of IDE2 and an additional 19 bp, could drive Fe-deficiency-inducible expression without IDE1 throughout almost the entire root. The principal modules of IDE1 and IDE2 were homologous. Sequences homologous to IDE1 were also found in many other Fe-deficiency-inducible promoters, including: nicotianamine aminotransferase (HvNAAT)-A, HvNAAT-B, nicotianamine synthase (HvNAS1), HvIDS3, OsNAS1, OsNAS2, OsIRT1, AtIRT1, and AtFRO2, suggesting the conservation of cis-acting elements in various genes and species. The identification of novel cis-acting elements, IDE1 and IDE2, will provide powerful tools to clarify the molecular mechanisms regulating Fe homeostasis in higher plants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
780-93
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:14675444-Base Sequence, pubmed-meshheading:14675444-Gene Expression Regulation, Enzymologic, pubmed-meshheading:14675444-Gene Expression Regulation, Plant, pubmed-meshheading:14675444-Hordeum, pubmed-meshheading:14675444-Iron, pubmed-meshheading:14675444-Mixed Function Oxygenases, pubmed-meshheading:14675444-Molecular Sequence Data, pubmed-meshheading:14675444-Mutagenesis, Insertional, pubmed-meshheading:14675444-Oligodeoxyribonucleotides, pubmed-meshheading:14675444-Plant Proteins, pubmed-meshheading:14675444-Plant Roots, pubmed-meshheading:14675444-Plants, Genetically Modified, pubmed-meshheading:14675444-Promoter Regions, Genetic, pubmed-meshheading:14675444-Sequence Alignment, pubmed-meshheading:14675444-Sequence Homology, Nucleic Acid, pubmed-meshheading:14675444-Tobacco
pubmed:year
2003
pubmed:articleTitle
Identification of novel cis-acting elements, IDE1 and IDE2, of the barley IDS2 gene promoter conferring iron-deficiency-inducible, root-specific expression in heterogeneous tobacco plants.
pubmed:affiliation
Laboratory of Plant Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
pubmed:publicationType
Journal Article