Source:http://linkedlifedata.com/resource/pubmed/id/14675193
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2003-12-16
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| pubmed:abstractText |
We describe a novel culture system for generating large numbers of murine skin-associated mast cells and distinguish their characteristics from bone marrow-derived cultured mast cells. Culture of day 16 fetal skin single cell suspensions in the presence of interleukin-3 and stem cell factor allowed expansion and maturation of mast cells in the presence of stromal cells. The average yield of mast cells after 2 wk was 7.3 million cells per fetus at a purity of 96%. These fetal skin-derived cultured mast cells increased their histamine content in a time-dependent manner to 3.6 pg per cell after 2 wk and 6.7 pg per cell after 4 wk. Phenotypic analyses revealed much greater expression of CD49b and CD81 and lesser expression of CD77 and CD102 on fetal skin-derived cultured mast cells as compared with bone marrow-derived cultured mast cells. These findings suggest a close similarity between fetal skin-derived cultured mast cells and freshly isolated cutaneous mast cells. Connective tissue mast cell characteristics of fetal skin-derived cultured mast cells were evidenced by: (1) their greater histamine content than bone marrow-derived cultured mast cells; (2) the presence of heparin; and (3) their degranulation in response to compound 48/80 and substance P. Importantly, fetal skin-derived cultured mast cells secreted greater amounts of interleukin-13 but much less MIP-1beta and interleukin-6 than bone marrow-derived cultured mast cells in response to ionomycin. Thus fetal skin-derived cultured mast cells have many characteristics distinct from bone marrow-derived cultured mast cells and can be used as a model of cutaneous mast cells to discern their functions.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monocyte Chemoattractant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgE,
http://linkedlifedata.com/resource/pubmed/chemical/p-Methoxy-N-methylphenethylamine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0022-202X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
121
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1425-32
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14675193-Age Factors,
pubmed-meshheading:14675193-Animals,
pubmed-meshheading:14675193-Cell Culture Techniques,
pubmed-meshheading:14675193-Cell Degranulation,
pubmed-meshheading:14675193-Cell Differentiation,
pubmed-meshheading:14675193-Cell Division,
pubmed-meshheading:14675193-Cells, Cultured,
pubmed-meshheading:14675193-Chemokine CCL4,
pubmed-meshheading:14675193-Fetus,
pubmed-meshheading:14675193-Heparin,
pubmed-meshheading:14675193-Histamine,
pubmed-meshheading:14675193-Interleukin-13,
pubmed-meshheading:14675193-Interleukin-6,
pubmed-meshheading:14675193-Macrophage Inflammatory Proteins,
pubmed-meshheading:14675193-Mast Cells,
pubmed-meshheading:14675193-Mice,
pubmed-meshheading:14675193-Mice, Inbred BALB C,
pubmed-meshheading:14675193-Mice, Inbred C57BL,
pubmed-meshheading:14675193-Monocyte Chemoattractant Proteins,
pubmed-meshheading:14675193-Phenotype,
pubmed-meshheading:14675193-RNA, Messenger,
pubmed-meshheading:14675193-Receptors, IgE,
pubmed-meshheading:14675193-Skin,
pubmed-meshheading:14675193-Stromal Cells,
pubmed-meshheading:14675193-p-Methoxy-N-methylphenethylamine
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| pubmed:year |
2003
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| pubmed:articleTitle |
Generation of a large number of connective tissue type mast cells by culture of murine fetal skin cells.
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| pubmed:affiliation |
Dermatology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
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| pubmed:publicationType |
Journal Article
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