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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2003-12-16
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pubmed:abstractText |
Human Aurora/Ipl1-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome alpha-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0300-8177
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
254
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-62
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pubmed:dateRevised |
2011-7-11
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pubmed:meshHeading |
pubmed-meshheading:14674694-Cell Cycle,
pubmed-meshheading:14674694-Cysteine Endopeptidases,
pubmed-meshheading:14674694-DNA, Complementary,
pubmed-meshheading:14674694-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:14674694-Glutathione Transferase,
pubmed-meshheading:14674694-HeLa Cells,
pubmed-meshheading:14674694-Humans,
pubmed-meshheading:14674694-Immunoblotting,
pubmed-meshheading:14674694-Mitosis,
pubmed-meshheading:14674694-Multienzyme Complexes,
pubmed-meshheading:14674694-Plasmids,
pubmed-meshheading:14674694-Precipitin Tests,
pubmed-meshheading:14674694-Proteasome Endopeptidase Complex,
pubmed-meshheading:14674694-Protein Binding,
pubmed-meshheading:14674694-Protein Biosynthesis,
pubmed-meshheading:14674694-Protein-Serine-Threonine Kinases,
pubmed-meshheading:14674694-Two-Hybrid System Techniques
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pubmed:year |
2003
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pubmed:articleTitle |
Human aurora-B binds to a proteasome alpha-subunit HC8 and undergoes degradation in a proteasome-dependent manner.
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pubmed:affiliation |
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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