Linked Life Data

14672804

Source:http://linkedlifedata.com/resource/pubmed/id/14672804

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-12-15
pubmed:abstractText
The rodent olfactory mucosa is characterized by a mosaic of gene expression that is exhibited among various cell types. Olfactory sensitivity in these animals is conveyed through odorant receptor families that are distinctly expressed within various subsets of the olfactory neuron population. Receptor neurons that express a particular class of odorant receptors exhibit bilaterally symmetric zones, which generally define their location within the nasal cavity. Less characterized are zonal expression profiles of proteins among non-neuronal cell types of the olfactory mucosa. In this study, we survey the expression of three glutathione S-transferase (GST) isozymes (alpha, mu, and pi) in the mouse olfactory mucosa and characterize the zonal expression of the mu isozyme. Immunohistochemistry and Western blot analysis of the GST mu isozyme reveal that the lateral olfactory turbinates I, Ib, II, IIb, and III display a greater intensity of expression for GST mu, in comparison to the dorsal and septal regions of the mucosa. GST alpha and pi isozymes do not display any distinct zonal organization in olfactory tissue of the adult mouse. When the general substrate 1-chloro-2-4-dinitrobenzene (CDNB) was used to assess GST activity within the olfactory tissue, the lateral turbinate regions displayed a higher level of activity when compared to dorsal or septal regions. Analysis of GST mu expression in prenatal and early postnatal olfactory tissue also reveals a zonal expression of the isozyme. We relate the significance of these findings to metabolic topography and olfactory chemosensory function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
995
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
151-7
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Zonal expression and activity of glutathione S-transferase enzymes in the mouse olfactory mucosa.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, College of Medicine, Howard University, 520 W St NW, Room 3430, Washington, DC 20059, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't