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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-12-11
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pubmed:abstractText |
We describe a technique to tag Drosophila proteins with GFP at their native genomic loci. This technique uses a new, small P transposable element (the Wee-P) that is composed primarily of the green fluorescent protein (GFP) sequence flanked by consensus splice acceptor and splice donor sequences. We demonstrate that insertion of the Wee-P can generate GFP fusions with native proteins. We further demonstrate that GFP-tagged proteins have correct subcellular localization and can be expressed at near-normal levels. We have used the Wee-P to tag genes with a wide variety of functions, including transmembrane proteins. A genetic analysis of 12 representative fusion lines demonstrates that loss-of-function phenotypes are not caused by the Wee-P insertion. This technology allows the generation of GFP-tagged reagents on a genome-wide scale with diverse potential applications.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-10021337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-10454621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-10471706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-10564276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-10716700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-10878606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-10890448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-11030623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-11156992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-11157986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-11395008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-11742088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-11810637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-1508718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-1905801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-2111451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-2462566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-2477306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-2509077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-2558049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-2852138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-8110464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-8244010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-8303295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-8344382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-8618928,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/14668392-9680312
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1433-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:14668392-Animals,
pubmed-meshheading:14668392-Artificial Gene Fusion,
pubmed-meshheading:14668392-Base Sequence,
pubmed-meshheading:14668392-DNA Primers,
pubmed-meshheading:14668392-DNA Transposable Elements,
pubmed-meshheading:14668392-Drosophila,
pubmed-meshheading:14668392-Drosophila Proteins,
pubmed-meshheading:14668392-Genomics,
pubmed-meshheading:14668392-Green Fluorescent Proteins,
pubmed-meshheading:14668392-Luminescent Proteins,
pubmed-meshheading:14668392-Recombinant Fusion Proteins,
pubmed-meshheading:14668392-Subcellular Fractions
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pubmed:year |
2003
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pubmed:articleTitle |
Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143-0448, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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