| pubmed-article:14668208 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C0001675 | lld:lifeskim |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C1882657 | lld:lifeskim |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C0215848 | lld:lifeskim |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C0475264 | lld:lifeskim |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:14668208 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:14668208 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:14668208 | pubmed:dateCreated | 2004-3-22 | lld:pubmed |
| pubmed-article:14668208 | pubmed:abstractText | The epididymal epithelium contributes to formation of a luminal fluid that is essential for the protection of spermatozoa from a variety of insults including changes in oxygen tension. A key regulator of the response to oxygen debt in many cells is hypoxia-inducible factor-1 (HIF-1). A transcription factor composed of alpha and beta subunits, HIF-1 activates genes that mediate oxygen homeostasis and cell survival pathways or trigger cell death responses. Previously we have shown that HIF-1alpha mRNA is expressed in the adult rat epididymis. Goals of this study were to determine whether HIF-1alpha protein is activated by ischemia in the rat epididymis, to determine whether epididymal HIF-1alpha mRNA expression is androgen dependent, and to identify epididymal cell types expressing HIF-1alpha and beta. Immunoblot analysis revealed that HIF-1alpha protein is primarily present in corpus and cauda of the normoxic epididymis and unaffected by ischemia, whereas HIF-1beta was detected equally in all regions and also unaffected by ischemia. HIF-1alpha mRNA expression in all regions was not affected by 15 days bilateral orchiectomy. Principal cells stained positive for HIF-1alpha by immunocytochemistry, with the epithelium of initial segment and caput epididymidis staining less intensely than corpus and cauda. HIF-1beta immunoreactivity was equally present in principal cells in all regions. Clear, narrow, and basal cells were unreactive for HIF-1alpha and beta. The presence of HIF-1 in normoxic epididymis and the regional distribution of HIF-1alpha suggests fundamental differences in how proximal and distal regions of the epididymis maintain oxygen homeostasis to protect the epithelium and spermatozoa from hypoxia. | lld:pubmed |
| pubmed-article:14668208 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14668208 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14668208 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14668208 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:14668208 | pubmed:issn | 0006-3363 | lld:pubmed |
| pubmed-article:14668208 | pubmed:author | pubmed-author:PalladinoM... | lld:pubmed |
| pubmed-article:14668208 | pubmed:author | pubmed-author:HermoLL | lld:pubmed |
| pubmed-article:14668208 | pubmed:author | pubmed-author:PowellJ DJD | lld:pubmed |
| pubmed-article:14668208 | pubmed:author | pubmed-author:KoramAA | lld:pubmed |
| pubmed-article:14668208 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14668208 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:14668208 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14668208 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14668208 | pubmed:pagination | 1121-30 | lld:pubmed |
| pubmed-article:14668208 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:meshHeading | pubmed-meshheading:14668208... | lld:pubmed |
| pubmed-article:14668208 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14668208 | pubmed:articleTitle | Expression and localization of hypoxia-inducible factor-1 subunits in the adult rat epididymis. | lld:pubmed |
| pubmed-article:14668208 | pubmed:affiliation | Department of Biology, Monmouth University, West Long Branch, New Jersey 07764, USA. mpalladi@monmouth.edu | lld:pubmed |
| pubmed-article:14668208 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14668208 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
