Linked Life Data

14668208

Source:http://linkedlifedata.com/resource/pubmed/id/14668208

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-3-22
pubmed:abstractText
The epididymal epithelium contributes to formation of a luminal fluid that is essential for the protection of spermatozoa from a variety of insults including changes in oxygen tension. A key regulator of the response to oxygen debt in many cells is hypoxia-inducible factor-1 (HIF-1). A transcription factor composed of alpha and beta subunits, HIF-1 activates genes that mediate oxygen homeostasis and cell survival pathways or trigger cell death responses. Previously we have shown that HIF-1alpha mRNA is expressed in the adult rat epididymis. Goals of this study were to determine whether HIF-1alpha protein is activated by ischemia in the rat epididymis, to determine whether epididymal HIF-1alpha mRNA expression is androgen dependent, and to identify epididymal cell types expressing HIF-1alpha and beta. Immunoblot analysis revealed that HIF-1alpha protein is primarily present in corpus and cauda of the normoxic epididymis and unaffected by ischemia, whereas HIF-1beta was detected equally in all regions and also unaffected by ischemia. HIF-1alpha mRNA expression in all regions was not affected by 15 days bilateral orchiectomy. Principal cells stained positive for HIF-1alpha by immunocytochemistry, with the epithelium of initial segment and caput epididymidis staining less intensely than corpus and cauda. HIF-1beta immunoreactivity was equally present in principal cells in all regions. Clear, narrow, and basal cells were unreactive for HIF-1alpha and beta. The presence of HIF-1 in normoxic epididymis and the regional distribution of HIF-1alpha suggests fundamental differences in how proximal and distal regions of the epididymis maintain oxygen homeostasis to protect the epithelium and spermatozoa from hypoxia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1121-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14668208-Animals, pubmed-meshheading:14668208-Aryl Hydrocarbon Receptor Nuclear Translocator, pubmed-meshheading:14668208-DNA-Binding Proteins, pubmed-meshheading:14668208-Epididymis, pubmed-meshheading:14668208-Hypoxia-Inducible Factor 1, alpha Subunit, pubmed-meshheading:14668208-Immunoblotting, pubmed-meshheading:14668208-Immunohistochemistry, pubmed-meshheading:14668208-Ischemia, pubmed-meshheading:14668208-Male, pubmed-meshheading:14668208-Orchiectomy, pubmed-meshheading:14668208-RNA, Messenger, pubmed-meshheading:14668208-Rats, pubmed-meshheading:14668208-Rats, Sprague-Dawley, pubmed-meshheading:14668208-Receptors, Aryl Hydrocarbon, pubmed-meshheading:14668208-Spermatozoa, pubmed-meshheading:14668208-Testosterone, pubmed-meshheading:14668208-Tissue Distribution, pubmed-meshheading:14668208-Transcription Factors
pubmed:year
2004
pubmed:articleTitle
Expression and localization of hypoxia-inducible factor-1 subunits in the adult rat epididymis.
pubmed:affiliation
Department of Biology, Monmouth University, West Long Branch, New Jersey 07764, USA. mpalladi@monmouth.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't