Linked Life Data

14662787

Source:http://linkedlifedata.com/resource/pubmed/id/14662787

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-12-9
pubmed:abstractText
Direct cooling of the testis results in the depletion of most germ cells in vivo. Germ cell-depleted testes are now commonly used to investigate spermatogenic regeneration and can serve as recipients for germ cell transplantation. The present study explored the effects of cooling rat testes on the depletion of endogenous germ cells, spermatogenic regeneration, and Sertoli cell function. Adult rat testes were cooled with iced Ringer's solution for 60 minutes, which results in the initiation of apoptotic germ cell loss within 8 hours. Pachytene spermatocytes at stages XII-I were the cells most sensitive to cooling. In 46%-67% of seminiferous tubule cross-sections, only Sertoli cells remained in the cooled testes 3-10 weeks after treatment. Germ cell loss was accompanied by a significant decrease in circulating inhibin B and an increase in follicle-stimulating hormone concentrations, which indicated a change in Sertoli cell function. Quantitative analysis of mRNA expression associated with apoptotic signals showed no significant uniform changes among the cooled testes, although some individuals had a distinct up-regulation of FAS mRNA at 24 hours. Attempts to use the cooled testes as recipient testes for mouse-to-rat germ cell transplantation were undertaken, but none of the mouse germ cells transplanted into the testes 15-34 days after cooling appeared to have undergone spermatogenesis 64-92 days after transplantation. These data suggest that modifications to Sertoli cell function resulting from testicular cooling create an environment that is unable to support spermatogenesis by donor germ cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0196-3635
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-68
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:14662787-Animals, pubmed-meshheading:14662787-Apoptosis, pubmed-meshheading:14662787-Body Temperature, pubmed-meshheading:14662787-Caspase 3, pubmed-meshheading:14662787-Caspases, pubmed-meshheading:14662787-Cell Transplantation, pubmed-meshheading:14662787-Cold Temperature, pubmed-meshheading:14662787-Hypothermia, Induced, pubmed-meshheading:14662787-Male, pubmed-meshheading:14662787-Organ Size, pubmed-meshheading:14662787-Proto-Oncogene Proteins, pubmed-meshheading:14662787-Proto-Oncogene Proteins c-bcl-2, pubmed-meshheading:14662787-RNA, Messenger, pubmed-meshheading:14662787-Rats, pubmed-meshheading:14662787-Rats, Sprague-Dawley, pubmed-meshheading:14662787-Sertoli Cells, pubmed-meshheading:14662787-Spermatogonia, pubmed-meshheading:14662787-Testis, pubmed-meshheading:14662787-Testosterone, pubmed-meshheading:14662787-bcl-2-Associated X Protein, pubmed-meshheading:14662787-bcl-X Protein
pubmed:articleTitle
Functional analysis of the cooled rat testis.
pubmed:affiliation
Department of Zoology, University of Melbourne, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't