Linked Life Data

14660381

Source:http://linkedlifedata.com/resource/pubmed/id/14660381

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2003-12-8
pubmed:abstractText
Xylella fastidiosa causes Pierce's disease of grapevine as well as several other major agricultural diseases but is a benign endophyte in most host plants. X. fastidiosa colonizes the xylem vessels of host plants and is transmitted by xylem sap-feeding insect vectors. To understand better the pattern of host colonization and its relationship to disease, we engineered X. fastidiosa to express a green fluorescent protein (Gfp) constitutively and performed confocal laser-scanning microscopic analysis of colonization in a susceptible host, Vitis vinifera. In symptomatic leaves, the fraction of vessels colonized by X. fastidiosa was fivefold higher than in nearby asymptomatic leaves. The fraction of vessels completely blocked by X. fastidiosa colonies increased 40-fold in symptomatic leaves and was the feature of colonization most dramatically linked to symptoms. Therefore, the extent of vessel blockage by bacterial colonization is highly likely to be a crucial variable in symptom expression. Intriguingly, a high proportion (>80%) of colonized vessels were not blocked in infected leaves and instead had small colonies or solitary cells, suggesting that vessel blockage is not a colonization strategy employed by the pathogen but, rather, a by-product of endophytic colonization. We present evidence for X. fastidiosa movement through bordered pits to neighboring vessels and propose that vessel-to-vessel movement is a key colonization strategy whose failure results in vessel plugging and disease.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-10910347, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-11059491, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-11386365, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-12023085, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-12200328, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-12205291, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-12529513, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-12533478, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-12757950, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-15012538, http://linkedlifedata.com/resource/pubmed/commentcorrection/14660381-4855307
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7319-27
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Use of a green fluorescent strain for analysis of Xylella fastidiosa colonization of Vitis vinifera.
pubmed:affiliation
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't