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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2003-12-5
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pubmed:abstractText |
We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome.A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology.Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and (2) the shortest-path analysis method. These analyses identified a number of gene groups, and further investigation revealed that the genes that were grouped together had close biological relationships. The artificial transcription factor-based random genome perturbation method thus provides a novel functional genomic tool for annotation and classification of genes in the human genome and those of many other organisms.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-10066362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-10344738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-10471706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-10591207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-10908728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-10910050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-10929718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11318608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11481453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11526084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11535131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11694880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11709701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11743205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11849947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-11884620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-12049748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-12072436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-12109515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-12140549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-12196633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-12570857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-1568479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-1700475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-2460224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-7508822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-7677242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-7780739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-8624732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-8706788,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/14656973-9872747
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1088-9051
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2708-16
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:14656973-Antigens, Neoplasm,
pubmed-meshheading:14656973-Cell Line,
pubmed-meshheading:14656973-Computational Biology,
pubmed-meshheading:14656973-DNA-Binding Proteins,
pubmed-meshheading:14656973-Gene Expression Profiling,
pubmed-meshheading:14656973-Gene Expression Regulation,
pubmed-meshheading:14656973-Genetic Linkage,
pubmed-meshheading:14656973-Genome, Human,
pubmed-meshheading:14656973-Humans,
pubmed-meshheading:14656973-Melanoma,
pubmed-meshheading:14656973-Multigene Family,
pubmed-meshheading:14656973-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:14656973-Recombinant Proteins,
pubmed-meshheading:14656973-Transcription Factors,
pubmed-meshheading:14656973-Zinc Fingers
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pubmed:year |
2003
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pubmed:articleTitle |
Toward a functional annotation of the human genome using artificial transcription factors.
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pubmed:affiliation |
ToolGen, Inc., Yuseong-gu, Daejeon, South Korea, 305-390.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Validation Studies
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