|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2003-12-3
|
|
pubmed:abstractText |
Human cord blood-derived mast cells (HCMC) grown in medium with serum and recombinant human stem cell factor (rhSCF) with or without interleukin (IL)-6 are less mature than human skin mast cells (HSMC). We found that c-kit-positive HCMC cultured for 8-10 weeks with rhSCF in serum-free medium became sensitive to basic secretatogues and expressed the serine protease, chymase, which is preferentially expressed in HSMC. The HCMC release beta-hexosaminidase (beta-HEX) within 1 min of stimulation with compound 48/80 or substance P, and release was suppressed by pertussis toxin. Approximately 34% of the HCMC in the serum-free culture stained positively with chymase antibody. Chymase and c-kit levels, and responsiveness to basic secretagogues, increased substantially after an additional 2 weeks in a serum-free environment with rhIL-6 and rhSCF. Moreover, Fc(epsilon)RI-dependent activation of the HCMC resulted in induction of cytokines and cyclooxygenase-2. These results show that HCMC can differentiate into a phenotype morphologically and functionally similar to HSMC if exposed to SCF in serum-free medium.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chymases,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Substance P,
http://linkedlifedata.com/resource/pubmed/chemical/beta-N-Acetylhexosaminidases,
http://linkedlifedata.com/resource/pubmed/chemical/p-Methoxy-N-methylphenethylamine
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
1016-8478
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
31
|
|
pubmed:volume |
16
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
154-60
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:14651255-Cell Degranulation,
pubmed-meshheading:14651255-Cells, Cultured,
pubmed-meshheading:14651255-Chymases,
pubmed-meshheading:14651255-Culture Media, Serum-Free,
pubmed-meshheading:14651255-Cyclooxygenase 2,
pubmed-meshheading:14651255-Cytokines,
pubmed-meshheading:14651255-Female,
pubmed-meshheading:14651255-Fetal Blood,
pubmed-meshheading:14651255-Humans,
pubmed-meshheading:14651255-Interleukin-6,
pubmed-meshheading:14651255-Isoenzymes,
pubmed-meshheading:14651255-Mast Cells,
pubmed-meshheading:14651255-Membrane Proteins,
pubmed-meshheading:14651255-Pertussis Toxin,
pubmed-meshheading:14651255-Pregnancy,
pubmed-meshheading:14651255-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:14651255-Recombinant Proteins,
pubmed-meshheading:14651255-Serine Endopeptidases,
pubmed-meshheading:14651255-Stem Cell Factor,
pubmed-meshheading:14651255-Substance P,
pubmed-meshheading:14651255-beta-N-Acetylhexosaminidases,
pubmed-meshheading:14651255-p-Methoxy-N-methylphenethylamine
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Degranulation and cytokine expression in human cord blood-derived mast cells cultured in serum-free medium with recombinant human stem cell factor.
|
|
pubmed:affiliation |
College of Pharmacy, Yeungnam University, Gyongsan 712-749, Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
