Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-1-15
pubmed:abstractText
1. The effect of the calmodulin inhibitor N-(6-aminohexyl)-5-chloro-1-naphthalenesulphonamide (W7; 10 microM) on intracellular [Ca2+] ([Ca2+]i) and [H+], and on contraction, has been studied in myocytes isolated from the ventricles of rat hearts. [Ca2+]i and [H+] were monitored using the fluorescent dyes Fura-2 and 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) respectively. 2. W7 decreased the size of both the Fura-2 fluorescence (a function of [Ca2+]i) transient and twitch, but had no effect on their time course. 3. The decrease in the size of the Fura-2 fluorescence transient in the presence of W7 was accompanied by a decrease in the increase of Fura-2 fluorescence that could be elicited by releasing Ca2+ from the sarcoplasmic reticulum using 10 mM-caffeine. 4. There was a decrease in the apparent sensitivity of the contractile proteins to Ca2+ in the presence of W7 which may account, in part, for the decrease in the twitch observed in the presence of W7. 5. Test beats were interpolated at different test intervals after a train of steady-state contractions. Mechanical restitution curves were constructed by plotting the size of the test beat against the test interval. Both the size and the duration of the twitch increased as the test interval was prolonged. W7 slowed this mechanical restitution but had no effect on the changes in the duration of the twitch. 6. Intracellular pH was not altered by W7. 7. These results are discussed in terms of the known actions of calmodulin and W7.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-1886064, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-1890639, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-1984873, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2086772, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2100313, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-212065, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2184764, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2193525, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-227448, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2434495, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2447792, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2522833, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2536303, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2537030, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2580043, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2580044, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-2943284, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-3156859, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-3204364, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-3217241, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-3225553, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-3656151, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-3981128, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-6121798, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-6234308, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-6243188, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-6247638, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-6257283, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-6310131, http://linkedlifedata.com/resource/pubmed/commentcorrection/1464834-7274230
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:volume
453
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
385-400
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
The effect of a calmodulin inhibitor on intracellular [Ca2+] and contraction in isolated rat ventricular myocytes.
pubmed:affiliation
Department of Physiology, University of Leeds.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't