Source:http://linkedlifedata.com/resource/pubmed/id/14646593
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2003-12-3
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| pubmed:databankReference | |
| pubmed:abstractText |
We report here the isolation, characterization on genomic structure and expression of the D. melanogaster homolog of human parkin. The 2,122 bp parkin gene sequence contains six exons that form a 1,449 bp transcript encoding a protein of 482 amino acids. 151 bp of 5' and 112 bp of 3' untranslated regions were identified by a combination of 5'-RACE/primer extension and 3'-RACE, respectively. The 5' UTR contains three transcription initiation sites. Neither a classical TATA nor a CAAT box was found in the putative promoter sequence. However, binding sites for AhR-Arnt, AP4, NF1 and GATA transcription factors were identified. Transient transfection analysis of the 5' UTR confirmed its promoter activity in HEK 293 cells and SH-SY5Y neuronal cells using a dual luciferase reporting system. The amino acid sequence of D. melanogaster Parkin exhibits 42%, 43% and 43% identity to that of human, mouse and rat, respectively, representing a 54 kDa protein band via western blot analysis. It shows a high degree of conservation in the Ubiquitin-like domain at the N-terminus (34%), the In-Between RING finger domains (IBR, 65-69%), and the RING finger domains at the C-terminus (56-57%). The expression pattern of D. melanogaster parkin varies during the developmental stages, with the highest expression in the adult stage as measured by competitive RT-PCR. From immunostainings of the embryo, D. melanogaster parkin was expressed slightly higher in the central nervous system (brain and nerve cord) during the late embryonic stage.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
1226-3613
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
31
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| pubmed:volume |
35
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
393-402
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:14646593-Amino Acid Sequence,
pubmed-meshheading:14646593-Animals,
pubmed-meshheading:14646593-Base Sequence,
pubmed-meshheading:14646593-Cell Line,
pubmed-meshheading:14646593-Cloning, Molecular,
pubmed-meshheading:14646593-Conserved Sequence,
pubmed-meshheading:14646593-Drosophila Proteins,
pubmed-meshheading:14646593-Drosophila melanogaster,
pubmed-meshheading:14646593-Exons,
pubmed-meshheading:14646593-Gene Expression Regulation, Developmental,
pubmed-meshheading:14646593-Genomics,
pubmed-meshheading:14646593-Humans,
pubmed-meshheading:14646593-Introns,
pubmed-meshheading:14646593-Molecular Sequence Data,
pubmed-meshheading:14646593-Promoter Regions, Genetic,
pubmed-meshheading:14646593-RNA, Messenger,
pubmed-meshheading:14646593-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14646593-Sequence Alignment,
pubmed-meshheading:14646593-Sequence Homology, Amino Acid,
pubmed-meshheading:14646593-Transcription Initiation Site,
pubmed-meshheading:14646593-Ubiquitin-Protein Ligases
|
| pubmed:year |
2003
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| pubmed:articleTitle |
Genomic organization and expression of parkin in Drosophila melanogaster.
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| pubmed:affiliation |
Department of Biological Science, College of Natural Science, Ewha Womans University, Seoul 120-750, Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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