Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2003-12-3
pubmed:abstractText
Although the methodology of molecular microscopy has enormous potential, it is time consuming and labor intensive. The techniques required to produce a three-dimensional (3D) electron density map of a macromolecular structure normally require manual operation of an electron microscope by a skilled operator and manual supervision of the sometimes complex software needed for analysis and calculation of 3D maps. Systems to automate the process of data acquisition from an electron microscope are being developing and these systems are being integrated with specimen handling operations and post acquisition data processing. Here, the current performance of our existing systems and the future challenges involved in substantially improving both the sustained throughput and the yield of automated data collection and analysis are reported.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0909-0495
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:14646141-Algorithms, pubmed-meshheading:14646141-Biopolymers, pubmed-meshheading:14646141-Comovirus, pubmed-meshheading:14646141-Equipment Failure Analysis, pubmed-meshheading:14646141-Feasibility Studies, pubmed-meshheading:14646141-Hemocyanin, pubmed-meshheading:14646141-Image Enhancement, pubmed-meshheading:14646141-Image Interpretation, Computer-Assisted, pubmed-meshheading:14646141-Imaging, Three-Dimensional, pubmed-meshheading:14646141-Macromolecular Substances, pubmed-meshheading:14646141-Microscopy, Electron, pubmed-meshheading:14646141-Models, Molecular, pubmed-meshheading:14646141-Molecular Conformation, pubmed-meshheading:14646141-Robotics, pubmed-meshheading:14646141-Specimen Handling, pubmed-meshheading:14646141-Systems Integration, pubmed-meshheading:14646141-Tobacco Mosaic Virus
pubmed:year
2004
pubmed:articleTitle
Rapid routine structure determination of macromolecular assemblies using electron microscopy: current progress and further challenges.
pubmed:affiliation
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Evaluation Studies