Linked Life Data

14645903

Source:http://linkedlifedata.com/resource/pubmed/id/14645903

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 12
pubmed:dateCreated
2003-12-3
pubmed:databankReference
pubmed:abstractText
In an effort to define novel cellular factors regulating human immunodeficiency virus type 1 (HIV-1) replication, a differential display analysis has been performed on endogenously infected cells stimulated with the HIV-suppressive immunomodulator Murabutide. In this study, the cloning and identification of a Murabutide-downregulated gene, named RH116, bearing classical motifs that are characteristic of the DExH family of RNA helicases, are reported. The 116 kDa encoded protein shares 99.9 % similarity with MDA-5, an inducible RNA helicase described recently. Ectopic expression of RH116 in HeLa-CD4 cells inhibited cell growth and cell proliferation but had no measurable effect on programmed cell death. RH116 presented steady state cytoplasmic localization and could translocate to the nucleus following HIV-1 infection. Moreover, the endogenous expression of RH116, at both the transcript and protein levels, was found to be considerably upregulated after infection. Overexpression of RH116 in HIV-1-infected HeLa-CD4 cells also resulted in a dramatic increase in the level of secreted viral p24 protein. This enhancement in virus replication did not stem from upregulated proviral DNA levels but correlated with increased unspliced and singly spliced viral mRNA transcripts. These findings implicate RH116 in the regulation of HIV-1 replication and point to an apoptosis-independent role for this novel helicase in inducing cell growth arrest.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3215-25
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14645903-Acetylmuramyl-Alanyl-Isoglutamine, pubmed-meshheading:14645903-Adjuvants, Immunologic, pubmed-meshheading:14645903-Amino Acid Motifs, pubmed-meshheading:14645903-Anti-HIV Agents, pubmed-meshheading:14645903-Apoptosis, pubmed-meshheading:14645903-CD4-Positive T-Lymphocytes, pubmed-meshheading:14645903-Cell Survival, pubmed-meshheading:14645903-Cloning, Molecular, pubmed-meshheading:14645903-DEAD-box RNA Helicases, pubmed-meshheading:14645903-DNA, Complementary, pubmed-meshheading:14645903-HIV Core Protein p24, pubmed-meshheading:14645903-HIV-1, pubmed-meshheading:14645903-HeLa Cells, pubmed-meshheading:14645903-Humans, pubmed-meshheading:14645903-Molecular Sequence Data, pubmed-meshheading:14645903-Molecular Weight, pubmed-meshheading:14645903-RNA Helicases, pubmed-meshheading:14645903-Sequence Homology, Amino Acid, pubmed-meshheading:14645903-U937 Cells, pubmed-meshheading:14645903-Virus Replication
pubmed:year
2003
pubmed:articleTitle
A novel cellular RNA helicase, RH116, differentially regulates cell growth, programmed cell death and human immunodeficiency virus type 1 replication.
pubmed:affiliation
Laboratoire d'Immunologie Moléculaire de l'Infection et de l'Inflammation, Institut Pasteur de Lille, 1 Rue du Professeur Calmette, BP 245, 59019 Lille Cedex, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't