Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-2-17
pubmed:abstractText
Fibroblast growth factor 2 (FGF-2) is a pro-angiogenic mediator that is secreted by both normal and neoplastic cells. Intriguingly, FGF-2 has been shown to be exported by an endoplasmic reticulum/Golgi-independent pathway; however, the molecular machinery mediating this process has remained elusive. Here we introduce a novel in vitro system that functionally reconstitutes FGF-2 secretion. Based on affinity-purified plasma membrane inside-out vesicles, we demonstrate post-translational membrane translocation of FGF-2 as shown by protease protection experiments. This process is blocked at low temperature but apparently does not appear to be driven by ATP hydrolysis. FGF-2 membrane translocation occurs in a unidirectional fashion requiring both integral and peripheral membrane proteins. These findings provide direct evidence that FGF-2 secretion is based on its direct translocation across the plasma membrane of mammalian cells. When galectin-1 and macrophage migration inhibitory factor, other proteins exported by unconventional means, were analyzed for translocation into plasma membrane inside-out vesicles, galectin-1 was found to be transported as efficiently as FGF-2. By contrast, migration inhibitory factor failed to traverse the membrane of inside-out vesicles. These findings establish the existence of multiple distinct secretory routes that are operational in the absence of a functional endoplasmic reticulum/Golgi system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6244-51
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14645213-Adenosine Triphosphate, pubmed-meshheading:14645213-Animals, pubmed-meshheading:14645213-Antigens, CD8, pubmed-meshheading:14645213-CHO Cells, pubmed-meshheading:14645213-Cell Membrane, pubmed-meshheading:14645213-Cricetinae, pubmed-meshheading:14645213-Cytosol, pubmed-meshheading:14645213-Detergents, pubmed-meshheading:14645213-Endoplasmic Reticulum, pubmed-meshheading:14645213-Fibroblast Growth Factor 2, pubmed-meshheading:14645213-Galectin 1, pubmed-meshheading:14645213-Golgi Apparatus, pubmed-meshheading:14645213-Hydrolysis, pubmed-meshheading:14645213-Macrophage Migration-Inhibitory Factors, pubmed-meshheading:14645213-Microscopy, Electron, pubmed-meshheading:14645213-Models, Biological, pubmed-meshheading:14645213-Protein Transport, pubmed-meshheading:14645213-Sodium Dodecyl Sulfate, pubmed-meshheading:14645213-Temperature, pubmed-meshheading:14645213-Time Factors
pubmed:year
2004
pubmed:articleTitle
Unconventional secretion of fibroblast growth factor 2 is mediated by direct translocation across the plasma membrane of mammalian cells.
pubmed:affiliation
Heidelberg University Biochemistry Center, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't