Linked Life Data

14641175

Source:http://linkedlifedata.com/resource/pubmed/id/14641175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2003-12-3
pubmed:abstractText
The facultative intracellular bacterial pathogen Listeria monocytogenes is capable of replicating within a broad range of host cell types and host species. We report here the establishment of the fruit fly Drosophila melanogaster as a new model host for the exploration of L. monocytogenes pathogenesis and host response to infection. Listeria monocytogenes was capable of establishing lethal infections in adult fruit flies and larvae with extensive bacterial replication occurring before host death. Bacteria were found in the cytosol of insect phagocytic cells, and were capable of directing host cell actin polymerization. Bacterial gene products necessary for intracellular replication and cell-to-cell spread within mammalian cells were similarly found to be required within insect cells, and although previous work has suggested that L. monocytogenes virulence gene expression requires temperatures above 30 degrees C, bacteria within insect cells were found to express virulence determinants at 25 degrees C. Mutant strains of Drosophila that were compromised for innate immune responses demonstrated increased susceptibility to L. monocytogenes infection. These data indicate L. monocytogenes infection of fruit flies shares numerous features of mammalian infection, and thus that Drosophila has the potential to serve as a genetically tractable host system that will facilitate the analysis of host cellular responses to L. monocytogenes infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1462-5814
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
901-11
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:14641175-Actins, pubmed-meshheading:14641175-Animals, pubmed-meshheading:14641175-Bacterial Proteins, pubmed-meshheading:14641175-Cell Line, pubmed-meshheading:14641175-Colony Count, Microbial, pubmed-meshheading:14641175-Cytosol, pubmed-meshheading:14641175-DNA-Binding Proteins, pubmed-meshheading:14641175-Drosophila Proteins, pubmed-meshheading:14641175-Drosophila melanogaster, pubmed-meshheading:14641175-Gene Deletion, pubmed-meshheading:14641175-Genes, Bacterial, pubmed-meshheading:14641175-Immunity, Innate, pubmed-meshheading:14641175-Listeria monocytogenes, pubmed-meshheading:14641175-Listeriosis, pubmed-meshheading:14641175-Mutagenesis, Insertional, pubmed-meshheading:14641175-Phagocytes, pubmed-meshheading:14641175-Temperature, pubmed-meshheading:14641175-Transcription Factors, pubmed-meshheading:14641175-Virulence Factors
pubmed:year
2003
pubmed:articleTitle
Exploration of host-pathogen interactions using Listeria monocytogenes and Drosophila melanogaster.
pubmed:affiliation
Department of Pathobiology, University of Washington, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.