Linked Life Data

14640738

Source:http://linkedlifedata.com/resource/pubmed/id/14640738

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2003-12-3
pubmed:abstractText
We describe a new approach to measuring DNA hybridization using surface plasmon-coupled emission (SPCE). Excited fluorophores are known to couple with surface oscillations of electrons in thin metal films, typically 50 nm thick silver on a glass prism. These surface plasmons then radiate into the glass at a sharply defined angle determined by the emission wavelength and the optical properties of the glass and metal. This radiation has the same spectral profile as the emission spectrum of the fluorophores. We studied the emission due to Cy3-labeled DNA oligomers bound to complementary unlabeled oligomers which were themselves bound to the metal surface. Hybridization resulted in SPCE due to Cy3-DNA into the prism. Directional SPCE was observed whether the sample was illuminated from the sample side or through the glass substrate at the surface plasmon angle for the excitation wavelength. A large fraction of the total potential emission is coupled to the surface plasmons resulting in improved sensitivity. When illuminated through the prism at the surface plasmon angle, the sensitivity is increased due to the enhanced intensity of the resonance evanescent field. It is known that SPCE depends on proximity to the silver surface. As a result, changes in emission intensity are observed due to fluorophore localization even if hybridization does not affect the quantum yield of the fluorophore. The use of SPCE resulted in suppression of interfering emission from a noncomplementary Cy5-DNA oligomers due to weaker coupling of the more distant fluorophores with the surface plasmons. We expect SPCE to have numerous applications to nucleic acid analysis and for the measurement of bioaffinity reactions.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/EB-000682, http://linkedlifedata.com/resource/pubmed/grant/EB-00981, http://linkedlifedata.com/resource/pubmed/grant/HG-002655, http://linkedlifedata.com/resource/pubmed/grant/P41 RR008119-13, http://linkedlifedata.com/resource/pubmed/grant/P41 RR008119-14, http://linkedlifedata.com/resource/pubmed/grant/R01 EB000682-03, http://linkedlifedata.com/resource/pubmed/grant/R01 EB000682-04, http://linkedlifedata.com/resource/pubmed/grant/R01 EB000682-05, http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-02, http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-03, http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-04A1, http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-05, http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-06, http://linkedlifedata.com/resource/pubmed/grant/R21 EB000981-01, http://linkedlifedata.com/resource/pubmed/grant/R21 EB000981-02, http://linkedlifedata.com/resource/pubmed/grant/RR-08119
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0003-2700
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6629-33
pubmed:dateRevised
2011-5-17
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
DNA hybridization using surface plasmon-coupled emission.
pubmed:affiliation
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.