Linked Life Data

14639101

Source:http://linkedlifedata.com/resource/pubmed/id/14639101

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-11-25
pubmed:abstractText
SUMMARY: The authors have identified 37 proteins in the whole-cell extracts from human monoblastic leukemia (U937) cell and macrophage-derived foam cell. The in vitro foam cell model was established by incubating the human U937 cells with oxidized low-density lipoprotein. The global changes in protein expressions between U937 foam cell and normal U937 cells were measured with two-dimensional polyacrylamide gel electrophoresis, and some proteins were trypsin-digested and then identified through tandem mass spectrometry after capillary liquid chromatography separation. Some of the identified proteins were validated via Internet links to the U937 proteomic map provided on the Expasy proteomics server. The experimental data can provide potential markers for atherosclerotic studies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0160-2446
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
782-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Proteomic studies of macrophage-derived foam cell from human U937 cell line using two-dimensional gel electrophoresis and tandem mass spectrometry.
pubmed:affiliation
Department of Chemistry, Fudan University, Shanghai, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't