|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2003-11-25
|
|
pubmed:abstractText |
Hippocalcin is a neuronal calcium sensor protein that possesses a Ca2+/myristoyl switch allowing it to translocate to membranes. Translocation of hippocalcin in response to increased cytosolic [Ca2+] was examined in HeLa cells expressing hippocalcin-enhanced yellow fluorescent protein (EYFP) to determine the dynamics and Ca2+ affinity of the Ca2+/myristoyl switch in living cells. Ca2+-free hippocalcin was freely diffusible, as shown by photobleaching and use of a photoactivable GFP construct. The translocation was dependent on binding of Ca2+ by EF-hands 2 and 3. Using photolysis of NP-EGTA, the maximal kinetics of translocation was determined (t1/2 = 0.9 s), and this was consistent with a diffusion driven process. Low intensity photolysis of NP-EGTA produced a slow [Ca2+] ramp and revealed that translocation of hippocalcin-EYFP initiated at around 180 nM and was half maximal at 290 nM. Histamine induced a reversible translocation of hippocalcin-EYFP. The data show that hippocalcin is a sensitive Ca2+ sensor capable of responding to increases in intracellular Ca2+ concentration over the narrow dynamic range of 200-800 nM free Ca2+.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10022960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10066733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10200278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10559922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10660296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10676964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10683417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-10723031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11006299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11115393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11343658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11493661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11830654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11832230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11836243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11886447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11910115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11964161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-11994284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-12034721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-12049938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-12196554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-12220869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-12228718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-12393897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-12445469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-1280427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-7630423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-7806504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-8101711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-8360179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-9162068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-9296500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-9515967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14638856-9712909
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/HPCA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hippocalcin,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/yellow fluorescent protein, Bacteria
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0021-9525
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
24
|
|
pubmed:volume |
163
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
715-21
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:14638856-Bacterial Proteins,
pubmed-meshheading:14638856-Binding Sites,
pubmed-meshheading:14638856-Calcium,
pubmed-meshheading:14638856-Calcium Signaling,
pubmed-meshheading:14638856-Calcium-Binding Proteins,
pubmed-meshheading:14638856-Cell Membrane,
pubmed-meshheading:14638856-Chelating Agents,
pubmed-meshheading:14638856-Cytoplasm,
pubmed-meshheading:14638856-HeLa Cells,
pubmed-meshheading:14638856-Hippocalcin,
pubmed-meshheading:14638856-Histamine,
pubmed-meshheading:14638856-Humans,
pubmed-meshheading:14638856-Luminescent Proteins,
pubmed-meshheading:14638856-Nerve Tissue Proteins,
pubmed-meshheading:14638856-Neurons,
pubmed-meshheading:14638856-Protein Structure, Tertiary,
pubmed-meshheading:14638856-Protein Transport,
pubmed-meshheading:14638856-Recombinant Fusion Proteins
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Dynamics and calcium sensitivity of the Ca2+/myristoyl switch protein hippocalcin in living cells.
|
|
pubmed:affiliation |
Physiological Laboratory, University of Liverpool, Liverpool L69 3BX, England, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
