|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2003-11-25
|
|
pubmed:abstractText |
Acetylcholine releases a non-prostanoid endothelium-derived hyperpolarizing factor (EDHF) and nitric oxide from physiological salt solution perfused rat mesenteric arteries. This study reports an impairment in EDHF-mediated vasodilation in deoxycorticosterone acetate (DOCA)-salt hypertensive versus control normotensive rats. Nitric oxide-mediated vasodilation to acetylcholine was not altered in the animals. We hypothesize that free radical species generated as by-products of arachidonic acid metabolism contribute to impaired EDHF-mediated dilation in DOCA-salt hypertension. With or without reduced nicotinamide adenine dinucleotide phosphate (NADPH) as co-factor, arterial microsomes generate free radical species upon incubation with arachidonic acid. The production of free radicals was significantly higher in DOCA-salt versus control rat microsomes, and was totally eliminated by addition of cyclooxygenase-2 inhibitors NS-398 or celecoxib at 30 microM. Treatment of DOCA-salt rats with tempol (an antioxidant; 15 mg/kg, i.p., 21 days) alleviates hypertension; improves acetylcholine-induced EDHF-mediated vasodilation in DOCA-salt rats, and decreases arachidonic acid-driven microsomal free radical production. Serum level of 8-isoprostanes is elevated in DOCA-salt hypertension versus control or sham-salt rats, and the increase was reversed by tempol treatment. These results show that EDHF-mediated dilation of rat mesenteric arteries is impaired in DOCA-salt induced hypertension. Our data also suggest that cyclooxygenase-2 mediates free radical production, and that free radicals modulate the EDHF-mediated vascular response in DOCA-salt induced hypertension.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-ethyl-2-benzimidazolinone,
http://linkedlifedata.com/resource/pubmed/chemical/8-epi-prostaglandin F2alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic N-Oxides,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Desoxycorticosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprost,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride, Dietary,
http://linkedlifedata.com/resource/pubmed/chemical/Spin Labels,
http://linkedlifedata.com/resource/pubmed/chemical/Vasoconstrictor Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/cirazoline,
http://linkedlifedata.com/resource/pubmed/chemical/endothelium-dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/tempol
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0014-2999
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
14
|
|
pubmed:volume |
481
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
91-100
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:14637180-Acetylcholine,
pubmed-meshheading:14637180-Animals,
pubmed-meshheading:14637180-Antioxidants,
pubmed-meshheading:14637180-Benzimidazoles,
pubmed-meshheading:14637180-Biological Factors,
pubmed-meshheading:14637180-Blood Pressure,
pubmed-meshheading:14637180-Calcium Channel Agonists,
pubmed-meshheading:14637180-Cyclic N-Oxides,
pubmed-meshheading:14637180-Cyclooxygenase Inhibitors,
pubmed-meshheading:14637180-Desoxycorticosterone,
pubmed-meshheading:14637180-Dinoprost,
pubmed-meshheading:14637180-Dose-Response Relationship, Drug,
pubmed-meshheading:14637180-Enzyme Inhibitors,
pubmed-meshheading:14637180-Free Radicals,
pubmed-meshheading:14637180-Hypertension,
pubmed-meshheading:14637180-Imidazoles,
pubmed-meshheading:14637180-Indomethacin,
pubmed-meshheading:14637180-Male,
pubmed-meshheading:14637180-Mesenteric Arteries,
pubmed-meshheading:14637180-Microsomes,
pubmed-meshheading:14637180-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:14637180-Nitric Oxide Synthase,
pubmed-meshheading:14637180-Rats,
pubmed-meshheading:14637180-Rats, Sprague-Dawley,
pubmed-meshheading:14637180-Sodium Chloride, Dietary,
pubmed-meshheading:14637180-Spin Labels,
pubmed-meshheading:14637180-Vasoconstrictor Agents,
pubmed-meshheading:14637180-Vasodilation,
pubmed-meshheading:14637180-Vasodilator Agents
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Tempol, an antioxidant, restores endothelium-derived hyperpolarizing factor-mediated vasodilation during hypertension.
|
|
pubmed:affiliation |
Department of Physiology and Biophysics, Health Sciences Center, A-1214, University of Louisville, Louisville, KY 40292, USA. asadea01@louisville.edu
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
|
