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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2003-11-25
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pubmed:databankReference |
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pubmed:abstractText |
The mammalian AP-2 family of transcription factors consists of four members, AP-2 alpha, AP-2 beta, AP-2 gamma and AP-2 delta, which play an important role in regulating gene expression during development and differentiation of multiple organs and tissues. The defining feature of the AP-2 family is a highly conserved carboxy-terminal basic helix-turn-helix domain that is involved in dimerization and sequence-specific DNA-binding. In this report, we use bioinformatics to identify both the mouse and human AP-2 epsilon, a fifth member of the AP-2 family. The predicted mouse and human AP-2 epsilon proteins consist of 442 amino acids and show a high level of sequence similarity with other AP-2 proteins in the DNA-binding and dimerization domain and weak similarity in the N-terminal activation domain. Northern blot analysis reveals that among the adult mouse tissues examined, AP-2 epsilon is highly expressed in skin tissue. The human AP-2 epsilon gene maps to chromosome 1p42, consists of seven exons spanning 23 kb and exhibits a genomic structure similar to other AP-2 family members. Human AP-2 epsilon mRNA is expressed in human skin and keratinocytes grown in culture. Finally, we show that recombinant AP-2 epsilon can bind to AP-2 binding sequences from keratin promoters in electrophoretic mobility shift assays. Our study establishes AP-2 epsilon as a novel member of the AP-2 family, and suggests that it may play an important role in skin biology.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tcfap2d protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tcfap2e protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-2,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
321
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-102
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:14636996-Amino Acid Sequence,
pubmed-meshheading:14636996-Animals,
pubmed-meshheading:14636996-Base Sequence,
pubmed-meshheading:14636996-Binding Sites,
pubmed-meshheading:14636996-Blotting, Northern,
pubmed-meshheading:14636996-Cell Line,
pubmed-meshheading:14636996-Cloning, Molecular,
pubmed-meshheading:14636996-DNA, Complementary,
pubmed-meshheading:14636996-DNA-Binding Proteins,
pubmed-meshheading:14636996-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:14636996-Gene Expression,
pubmed-meshheading:14636996-HeLa Cells,
pubmed-meshheading:14636996-Humans,
pubmed-meshheading:14636996-Keratinocytes,
pubmed-meshheading:14636996-Male,
pubmed-meshheading:14636996-Mice,
pubmed-meshheading:14636996-Molecular Sequence Data,
pubmed-meshheading:14636996-Multigene Family,
pubmed-meshheading:14636996-Oligonucleotides,
pubmed-meshheading:14636996-Protein Binding,
pubmed-meshheading:14636996-RNA, Messenger,
pubmed-meshheading:14636996-Sequence Alignment,
pubmed-meshheading:14636996-Sequence Analysis, DNA,
pubmed-meshheading:14636996-Sequence Homology, Amino Acid,
pubmed-meshheading:14636996-Skin,
pubmed-meshheading:14636996-Transcription Factor AP-2,
pubmed-meshheading:14636996-Transcription Factors
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pubmed:year |
2003
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pubmed:articleTitle |
Molecular cloning and characterization of AP-2 epsilon, a fifth member of the AP-2 family.
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pubmed:affiliation |
Department of Biochemistry, State University of New York at Buffalo, 3435 Main Street, 121 Farber Hall, Buffalo, NY 14214, USA.
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pubmed:publicationType |
Journal Article
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