Linked Life Data

14636994

Source:http://linkedlifedata.com/resource/pubmed/id/14636994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2003-11-25
pubmed:abstractText
In this study, the effects of 1 mM sodium nitrite, a reactive nitrogen species (RNS) generator, and 0.5 mM paraquat, which produces reactive oxygen species (ROS), on gene expression in the marine dinoflagellate species Pyrocystis lunula were investigated using microarrays containing 3500 complementary DNAs (cDNAs). A total of 246 differentially expressed genes were identified under these treatments: 204 genes were specifically regulated in response to nitrite and 37 genes specifically to paraquat. Only six genes showed a dependence on both nitrite and paraquat, indicating that the two agents act predominantly via distinct pathways. Although many of these redox-regulated genes encode proteins from a diverse range of functional categories, the majority of them (68%) represent novel sequences. Temporary abnormal spherical cells occurred in nitrite-treated cultures, but not in those exposed to paraquat, suggesting that this response involves a specific pathway triggered by RNS. The genes involved include one that encodes a transcription factor unique to dinoflagellates (HPl), and genes encoding proteins similar to those regulating developmental processes in plants and animals such as NYD-SP5, shaggy and calcium-dependent kinases, the COP9 signalosome complex, ubiquitin-related proteases and a metacaspase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
321
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-81
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Genome-wide analysis of redox-regulated genes in a dinoflagellate.
pubmed:affiliation
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138-2020, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't