Source:http://linkedlifedata.com/resource/pubmed/id/14634153
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
2003-11-24
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pubmed:databankReference | |
pubmed:abstractText |
We isolated a novel C9 position specific lipoxygenase (r9-LOX1) cDNA from developing rice seeds. The enzymatic features of r9-LOX1 resembled those of rice LOX-L3 known to be contained in rice germ and to have C9-specific LOX activity. However, the expression level of the r9-LOX1 gene was higher in imbibed seeds rather than developing seeds. A homology search against the rice nucleotide database revealed the r9-LOX1 gene to be on rice chromosome 3 (accession number AC093017). The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0032-0781
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1168-75
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pubmed:meshHeading |
pubmed-meshheading:14634153-Amino Acid Sequence,
pubmed-meshheading:14634153-Base Sequence,
pubmed-meshheading:14634153-Cloning, Molecular,
pubmed-meshheading:14634153-DNA, Complementary,
pubmed-meshheading:14634153-DNA Primers,
pubmed-meshheading:14634153-Escherichia coli,
pubmed-meshheading:14634153-Lipoxygenase,
pubmed-meshheading:14634153-Molecular Sequence Data,
pubmed-meshheading:14634153-Oryza sativa,
pubmed-meshheading:14634153-Recombinant Proteins,
pubmed-meshheading:14634153-Seeds,
pubmed-meshheading:14634153-Sequence Homology, Amino Acid
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pubmed:year |
2003
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pubmed:articleTitle |
A new 9-lipoxygenase cDNA from developing rice seeds.
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pubmed:affiliation |
Institute of Agricultural and Forest Engineering, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. koumno@akita-pu.ac.jp
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pubmed:publicationType |
Journal Article
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