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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2003-11-24
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pubmed:abstractText |
Survivin plays an important role in cancer development. We aim to show here that suppression of survivin expression or function by antisense and dominant-negative (DN) mutant can inhibit gastric cancer carcinogenesis and angiogenesis in vivo. Plasmid constructs expressing survivin antisense and DN mutant replacing the cysteine residue at amino acid 84 with alanine (Cys84Ala) were prepared and introduced into BCG-823 and MKN-45 gastric cancer cells to establish stable transfectants. We showed that both antisense and DN mutant stable transfectants exhibited abnormal morphology, with decreased cell growth and increased rate of spontaneous apoptosis and mitotic catastrophe. Furthermore, in nude mice xenografts, these cells exhibited decreased de novo gastric tumor formation and reduced development of angiogenesis. Results from these studies strongly suggest that survivin is a promising target for gastric cancer treatment.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0008-5472
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pubmed:author |
pubmed-author:CaseN BNB,
pubmed-author:ChanAnnie On-OnAO,
pubmed-author:CuiJian TaoJT,
pubmed-author:JiangShi HuSH,
pubmed-author:JiangXiao HuaXH,
pubmed-author:KungHsiang FuHF,
pubmed-author:LamShiu KumSK,
pubmed-author:LinMarie C MMC,
pubmed-author:LumChing TungCT,
pubmed-author:TuShui PingSP,
pubmed-author:WongBenjamin Chun-YuBC,
pubmed-author:WongWai ManWM,
pubmed-author:YangYiY,
pubmed-author:YuenMan FungMF,
pubmed-author:ZhuYan BoYB
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7724-32
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14633697-Adenocarcinoma,
pubmed-meshheading:14633697-Animals,
pubmed-meshheading:14633697-Apoptosis,
pubmed-meshheading:14633697-Cell Division,
pubmed-meshheading:14633697-Cell Line, Tumor,
pubmed-meshheading:14633697-DNA, Antisense,
pubmed-meshheading:14633697-Female,
pubmed-meshheading:14633697-Humans,
pubmed-meshheading:14633697-Inhibitor of Apoptosis Proteins,
pubmed-meshheading:14633697-Mice,
pubmed-meshheading:14633697-Mice, Inbred BALB C,
pubmed-meshheading:14633697-Mice, Nude,
pubmed-meshheading:14633697-Microtubule-Associated Proteins,
pubmed-meshheading:14633697-Neoplasm Proteins,
pubmed-meshheading:14633697-Neovascularization, Pathologic,
pubmed-meshheading:14633697-Stomach Neoplasms,
pubmed-meshheading:14633697-Transfection,
pubmed-meshheading:14633697-Xenograft Model Antitumor Assays
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pubmed:year |
2003
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pubmed:articleTitle |
Suppression of survivin expression inhibits in vivo tumorigenicity and angiogenesis in gastric cancer.
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pubmed:affiliation |
Department of Gastroenterology, Rui-jin Hospital, Shanghai Second Medical University, Shanghai, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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